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为了探讨下丘脑神经内分泌的突触调控机制，本文用电镜细胞化学与免疫电镜双标技术相结合的方法，研究了大鼠下丘脑室旁核神经元的多重神经支配。即先用６－ＯＨＤＡ损毁ＣＡ能神经末梢，再于振动切片上用包埋前免疫电镜法，分别以ＤＡＢ和ＴＡＢ为呈色剂先后对肽能（ＯＴ或ＳＰ）神经元和ＧＡＢＡ神经元进行双重标记。电镜观察结果表明：在下丘脑室旁核内存在肽能（ＯＴ、ＳＰ）和氨基酸（ＧＡＢＡ）能神经元及ＣＡ神经末梢；ＯＴ神经元通过轴一树突触接受ＧＡＢＡ和ＣＡ神经支配；ＳＰ神经元通过轴－树突触接受ＣＡ和ＳＰ神经支配；ＧＡＢＡ神经元则可通过轴－树突触接受ＯＴ、ＳＰ、ＣＡ和ＧＡＢＡ等神经支配。本研究首次得到了下丘脑神经元接受多重神经支配的超微结构证据，其意义在于为同时研究下丘脑神经内分泌活动的多种突触调控提供形态学依据。
In order to investigate the synaptic regulation mechanism of hypothalamic neuroendocrine, this paper studied the multiple innervation of neurons in hypothalamic paraventricular nucleus by a combination of electron microscopy and immunoelectron microscopy. That is, 6-OHDA was used to destroy the CA nerve endings first, and then on the vibrational sections by immunohistochemistry before embedding, respectively using DAB and TAB as the color former to conduct peptide energy (OT or SP) neurons and GABA neurons Double marker. Electron microscopy results showed that there were peptide energy (OT, SP) and amino acid (GABA) and CA nerve endings in hypothalamic paraventricular nucleus; OT neurons innervated by GABA and CA through axonal-dendritic synapses; Metaphages accept CA and SP innervation through axis-dendrites; GABA neurons innervate innervation of OT, SP, CA, and GABA through axonal-to-dendritic synapses. This study, for the first time, has obtained ultrastructural evidence of multiple innervation of hypothalamic neurons. The significance of this study is to provide a morphological basis for the simultaneous study of multiple synaptic modulation of hypothalamic neuroendocrine activity.