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目的 研究视网膜色素上皮(RPE)细胞内吞光感受器细胞外基质(IPM)内大分子可溶性物质的可能性及其机制。方法 采用荧光标记的甲醛作用后的血清白蛋白(F-FSA),作为清道夫受体配体的指示物,与组织块培养的猪RPE细胞共同孵育,用荧光显微镜和电子显微镜观察RPE细胞对F-FSA的内吞情况。结果 RPE细胞能够摄入F-FSA;这一作用随配体浓度增加而提高,随孵育时间延长而增强;相同或相似的配体之间存在竞争作用;F-FSA进入RPE细胞后,以微囊泡的形式存在丁细胞中。结论 RPE细胞能够摄人携带负电荷的大分子物质,这一作用是通过受体介导的内吞作用完成的,清道夫受体可能是介导这一作用的受体;受体介导的RPE细胞的内吞作用可能是IPM内大分子物质代谢的重要机制。(中华眼科杂志,2004,40:539-544)
Objective To investigate the possibility and mechanism of macromolecule soluble substances in the extracellular matrix (IPM) of endometriotic receptors in retinal pigment epithelium (RPE) cells. Methods Fluoro-labeled formaldehyde (F-FSA) was used as an indicator of scavenger receptor ligands and incubated with porcine RPE cells in tissue culture. Fluorescent and electron microscopy were used to observe the effect of RPE cell pairs Endocytosis of F-FSA. Results RPE cells were able to ingest F-FSA. This effect was enhanced with the increase of ligand concentration and increased with incubation time. There was competition between the same or similar ligands. After F-FSA entered RPE cells, Vesicles exist in the form of D-cells. Conclusions RPE cells can ingest negatively charged macromolecules, and this effect is mediated through receptor-mediated endocytosis. Scavenger receptors may be the mediators of this effect. Receptor-mediated Endocytosis of RPE cells may be an important mechanism of macromolecular metabolism in IPM. (Chinese Journal of Ophthalmology, 2004,40: 539-544)