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以耐旱性差异较大的两个亲本珍汕97B(ZS97B)和IRAT109构建的重组自交系(RIL)为试验材料,在正常水分条件和干旱胁迫[浓度为18%的聚乙二醇-6000(PEG-6000)模拟干旱]条件对水稻苗期苗高、根长、苗高生长速率、根长苗高比、叶卷曲进行QTL定位分析,共检测到24个相关的QTL,贡献率变幅在7.35%~39.30%。其中正常条件下检测到13个相关的QTL位点,分布在第1、2、3、5、6、10、12染色体上;干旱胁迫条件下检测到11个相关的QTL位点,分布在第1、3、5、7、10、12染色体上。2种条件下检测到的QTL位点差异很大,表明不同处理条件下相关性状的遗传机制不同。此外,在第1染色体上的RM472~RM104存在控制苗高、苗高生长速率、根长、根长苗高比多个性状的QTL,并且此区间在2种处理条件下能重复检测到控制苗高位点。
Two inbred lines (ZS97B) and IRAT109 (RIL), two parents with different drought tolerance, were used as experimental materials. Under the condition of normal water stress and drought stress [concentration of 18% (PEG-6000) simulated drought conditions, QTL mapping of rice seedling height, root length, seedling height growth, root length and shoot height and leaf curl were carried out. A total of 24 QTLs were detected, In the range of 7.35% ~ 39.30%. Among them, 13 related QTLs were detected under the normal conditions and distributed on chromosomes 1, 2, 3, 5, 6, 10 and 12. Under drought stress, 11 related QTLs were detected, 1, 3, 5, 7, 10, 12 chromosomes. The results showed that there were significant differences in the QTLs detected under the two conditions, indicating that the genetic mechanisms of the related traits were different under different treatment conditions. In addition, there were QTLs for controlling seedling height, seedling height growth rate, root length and root length seedling height ratio of RM472 ~ RM104 on chromosome 1, and the control seedling could be repeatedly detected under the two treatment conditions High point.