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葡萄糖酸钙对链霉素—蛋白(ST-BSA)致敏豚鼠过敏休克的保护作用的实验研究,结果对照组一般过敏反应发生率为27%,休克率为53%,死亡率为20%;保护组一般过敏反应率为13%,无休克和死亡发生。致敏豚鼠离体回肠实验结果,对照组肠管收缩高度为48.7±10.8mm,保护组为2.2±4.1mm,两组差异显著(P<0.01);ST-BSA致敏家兔血清异种动物PCA实验结果,对照组抗体滴度为1:4096,原血清蓝斑直径为35.9±1.9mm,保护组抗体滴度为1:512,原血清蓝斑直径为25.2±0.8mm,两组抗体滴度及蓝斑直径差异显著(P<0.01);大鼠腹腔肥大细胞脱颗粒实验结果,致敏对照组脱颗粒率为54.3±7.1%,保护组为17.5±6.8%,两组结果有明显差异(P<0.01)。提示葡萄糖酸钙对ST-BSA致敏豚鼠过敏休克有保护作用,其机理可能与抑制肥大细胞脱颗粒释放组胺等过敏介质有关。
The protective effect of calcium gluconate on streptomycin-protein (ST-BSA)-sensitized guinea pig allergy shocks resulted in a 27% incidence of allergic reactions in the control group, a shock rate of 53%, and a mortality rate of 20%; In the protection group, the general allergic reaction rate was 13%, and no shock and death occurred. The sensitized guinea pig isolated ileal test results, the control group the intestine contraction height of 48.7 ± 10.8mm, protection group was 2.2 ± 4.1mm, the difference between the two groups was significant (P <0.01); ST-BSA sensitized rabbits serum xenograft PCA experiment As a result, the antibody titer of the control group was 1:4096, the diameter of the original blood locus was 35.9±1.9 mm, the antibody titer of the protective group was 1:512, and the diameter of the original blood locus was 25.2±0.8 mm. The antibody titers of the two groups were as follows: The difference in the diameter of the blue lobe was significant (P<0.01). The experimental results of rat peritoneal mast cell degranulation showed that the degranulation rate of the sensitized control group was 54.3±7.1% and that of the protective group was 17.5±6.8%. There was a significant difference between the two groups (P <0.01). It is suggested that calcium gluconate can protect ST-BSA sensitized guinea pigs from allergic shock, and the mechanism may be related to the inhibition of mast cell degranulation and release of histamine and other allergic mediators.