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以中秆(180cm)千斤红为受体,矮秆(75c)粒息B为供体,采用花粉匀浆方法将粒息B的矮秆性状快速导入千斤红中,使后代出现矮秆的千斤红植株.花粉匀浆缓冲液分别用0.1M的NaH_2PO_4与Na_2HPO_4配制(pH7.2),使用前加入0.25M的蔗糖.开花时将粒息B的花粉放入研磨器中,加入约二倍于花粉的花粉匀浆缓冲液进行研磨,使花粉粒完全破碎.用毛笔沾取花粉匀浆涂抹千斤红的柱头后,再授千斤红的花粉.1985年1月在海南岛共处理5株千斤红,每株去雄200朵花,共收1000粒种于.同年春天在所内按穗行播种.结果H_1(第1代)出现了13株矮秆的千斤红,变异率为1.3%.成熟后因1株早衰死亡,实收12个单穗.同时播种了千斤红×粒息B的F_1作为对照.
In the stem (180cm) Thousands of red as the acceptor, dwarf (75c) Granulation B as a donor, the use of pollen homogenization method of dwarf traits of grain B quickly into the red, so that d Red plant pollen homogenate buffer were prepared with 0.1M NaH 2 PO 4 and Na 2 HPO 4 (pH 7.2), 0.25M sucrose was added prior to use when the pollen grain B pollen into the grinder, adding about twice Pollen pollen slurry homogenate grinding, the pollen grains completely broken.Wash the brush with pollen homogenate coated stigma stigma, and then awarded the red killer pollen .In January 1985 in Hainan Island, a total of five strains of red , 200 flowers per plant were excised, and a total of 1000 seeds were planted. In the same year spring, sowing was done on the ear rows. Results Thirteen dwarf stems were observed in H_1 (1st generation) with a mutation rate of 1.3% As a result of an early premature death, 12 single ears were harvested, and F_1, which was a killer × seed B, was also used as a control.