以有髯鸢尾“白与蓝”ד印度首领”杂交种子成熟胚为试材,采用组织培养法,研究了消毒方式、生长调节剂的种类与浓度对有髯鸢尾种胚成苗影响,以期为鸢尾育种提供理论依据。结果表明:成熟胚诱导最适培养基为MS+2.0mg·L~(-1) 6-BA+0.5mg·L~(-1) IBA,萌发率达86.6%,成苗率达85.0%;丛生芽在MS+4.0mg·L~(-1) 6-BA+0.5mg·L~(-1) IBA的培养基上增殖系数最大,为5.76;适宜的生根培养基为MS+0.7mg·L~(-1) PP333,接种后7d开始生根,生根率达83.33%;组培苗生根后无需练苗便可移栽至蛭石中,移栽成活率89.53%。 The method of tissue culture was used to study the disinfection methods, the types and concentrations of growth regulators on the embryos with the 髯 iris seed germination, and the mature embryos with 髯 iris “white and blue” × “Indian head” Miao affect, in order to provide a theoretical basis for iris breeding. The results showed that the optimal culture medium for induction of mature embryos was MS + 2.0 mg · L -1 6-BA + 0.5 mg · L -1 IBA with a germination rate of 86.6% and a seedling rate of 85.0%. The multiplication coefficient of clustered buds was the highest in medium with MS + 4.0 mg · L -1 6-BA + 0.5 mg · L -1 IBA, and the suitable rooting medium was MS + 0.7 mg · L -1 L ~ (-1) PP333. The rooting rate reached 83.33% at 7d after inoculation. The tissue culture seedlings could be transplanted into vermiculite without need of seedling after rooting, and the survival rate after transplanting was 89.53%.