论文部分内容阅读
用透射电镜及电子探针X—射线显微分析法研究了丁丙诺啡(Buprenorphine,BN)引起镇痛期间小鼠中脑导水管周围灰质区钙离子分布的改变。按照改进的Komnick方法,脑组织用含有2%焦锑酸钾的1%锇酸固定。实验结果表明,动物经腹腔注射BN(0.8mg/kg)30分钟后,在髓鞘、轴突、线粒体和细胞核中均可见到电子致密的沉淀颗粒,尤其在髓鞘的环状片层中形成大量的、密集的颗粒状沉淀。电子探针X—射线显微分析证实髓鞘中的沉淀颗粒含有元素钙,提示BN镇痛时髓鞘结合大量的钙离子,并且可能经过髓鞘的转运,钙离子进入轴突,贮存于线粒体中。本文讨论了在中枢神经系统中的钙离子转运的可能途径。
Transmission electron microscopy and electron probe X-ray microscopy were used to investigate the changes of calcium distribution in the periaqueductal gray zone of mice during buprenorphine (BN) -induced analgesia. According to the modified Komnick method, brain tissue is fixed with 1% osmium tetroxide containing 2% potassium pyroantimonate. The experimental results show that after 30 minutes of intraperitoneal injection of BN (0.8 mg / kg), electron-dense precipitated particles can be seen in myelin, axon, mitochondria and nucleus, especially in the annular layer of myelin Large, dense, granular deposits. Electron probe X-ray microscopy confirmed that the precipitated particles in myelin contained elemental calcium, suggesting that myelin sheath binds large amounts of calcium ions during BN analgesia and may transport through the myelin sheath into the axon and store in the mitochondria in. This article discusses the possible pathways for calcium transport in the central nervous system.