论文部分内容阅读
本文应用免疫细胞化学方法,结合图像分析仪,研究了缓激肽在脊髓腰段及L_(4-6)背根节的分布,以及坐骨神经切断后,它在相应的前角运动神经元的相对含量的变化规律.研究发现:缓激肽免疫阳性反应物分布于L4～L6背根节及腰骶髓灰质的第Ⅱ、Ⅲ、Ⅳ、Ⅸ层神经元及脊髓白质的神经胶质细胞和神经纤维.在神经损伤(切断坐骨神经)的研究中,相应脊髓前角运动神经元的缓激肽含量,在损伤后第15h减少,以后逐渐增多,在损伤后24h基本恢复到对照组水平,在第48h,其含量明显超过对照组,在第72h,继续维持在高位水平.
In this paper, the immunocytochemistry and image analyzer were used to study the distribution of bradykinin in the spinal cord lumbar and L_ (4-6) dorsal root ganglion, and after the sciatic nerve was cut off, the relative position of bradykinin in the corresponding anterior horn motor neurons The results showed that the bradykinin immunoreactive substances were distributed in the neurons of the second, the third, the fourth and the ninth layers of L4 ~ L6 dorsal root ganglion and lumbosacral gray matter and glial cells and nerves in the white matter of the spinal cord In the study of nerve injury (sciatic nerve sciatic nerve), the bradykinin content in the corresponding motor neurons in the anterior horn of the spinal cord decreased at 15 h after injury and gradually increased afterwards, and recovered to the control group at 24 h after injury. 48h, its content was significantly more than the control group, in 72h, continue to maintain high levels.