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本文报道一种快速而准确计数鼠肠内巴西日本圆线虫的方法。方法是将检查的实验大白鼠禁食过夜,杀死,取出全部小肠,将小肠纵剖,置于含有热的生理盐水(37℃)的培养皿中,放置室温中过夜。虫体游离肠组织移行到盐水中,在动物解剖后15～20小时内虫仍能存活。在这样长的时间内虫体的移行与宿主的免疫状况没有关系。此后将培养皿和内容物放在-20℃下冰冻,直至虫体计数时。计数前将培养皿中的内容物放入1,000毫升的量筒中融化和洗涤。用自来水通过接有一个增大水压的出水口的喷咀射入量筒,由于剧烈搅动,使各层肠组织与虫分离而悬
This article reports a rapid and accurate method for counting C. japonicus in the rat intestine. The test rats were fasted overnight, killed, all the small intestine was removed, and the small intestine was dissected longitudinally and placed in a petri dish containing hot physiological saline (37 ° C) and left at room temperature overnight. Insect free intestinal tissue migration to saline, animal 15 to 20 hours after the worm can survive. In such a long period of migration of parasites and host immune status has nothing to do. After that, the petri dish and the contents are frozen at -20 ° C until the number of parasites is counted. The contents of the dish were thawed and washed in a 1,000 ml graduated cylinder before counting. With tap water through a nozzle to increase water pressure nozzle into the cylinder, due to violent agitation, so that the layers of intestinal tissue separation and insects suspended