众所周知,RNA肿瘤病毒的转化基因(v-onc)来源于正常细胞的基因。这些基因在脊椎动物长期的进化中保存了下来。脊椎动物正常基因组中这些基因被称为原癌基因。它能够转换为有活性的癌基因(conc),以诱导和维持细胞的转化。一个v-onc基因细胞性的过分表达同诱导转化有关。另外,慢性白血病病毒可以通过将其基因组嵌入宿主DNA的方式,于经过较长期的潜伏期之后发生白血病。这里的嵌合点同c-onc逆向而行(upstream)的,因而引起这个c-onc基因转录的增加。由于c-onc基因很象它相应的病毒基因(v-onc),因此它的异常表达或不定期表达可能以某种方式诱发人类肿瘤。为了说明癌基因在肿瘤生长和分化中可能起的作用,作者采用凝胶Northern印迹技术,分析了各种 It is well-known that the transforming gene (v-onc) of the RNA tumor virus is derived from the genes of normal cells. These genes have been preserved in the long-term evolution of vertebrates. These genes in the normal genome of vertebrates are called proto-oncogenes. It can be converted to an active oncogene (conc) to induce and maintain cell transformation. The overexpression of a v-onc gene is associated with induced transformation. In addition, chronic leukemia viruses can develop leukemia after a longer incubation period by embedding their genome in the host DNA. The chimeric point here is upstream in the direction of c-onc, thus causing an increase in the transcription of this c-onc gene. Since the c-onc gene is much like its corresponding viral gene (v-onc), its abnormal expression or irregular expression may induce human tumors in some way. To illustrate the possible role of oncogenes in tumor growth and differentiation, the authors used gel Northern blotting techniques to analyze various