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目的建立C57BL6近交系小鼠前列腺癌原位移植瘤模型,观察在体前列腺癌生长转移的生物学特性。方法利用显微外科技术,将保持了完整组织结构的、移植于C57BL6近交系小鼠皮下的小鼠前列腺癌块,植于C57BL6近交系小鼠前列腺两腹侧叶之间,观察移植后肿瘤转移情况及小鼠的生存期。结果C57BL6进行RM-1肿瘤块原位移植术后,第15～20天,动物出现一般状态衰竭,体重下降明显,下腹可触及包块,全部为肿瘤占据,前列腺组织见不到。原位模型组大体见癌组织广泛地浸润周围盆腔脏器,其中淋巴结(16/16)、肺部(8/16)转移。两种模型荷瘤鼠生存时间原位模型组小鼠(17.0±3.5)d短于皮下模型组(33.0±5.6)d,两者相比差异有显著性。结论本模型可较好地模拟小鼠前列腺癌体内的自然生长状况,适合用于观察前列腺癌在体条件下的生物学特性。
Objective To establish C57BL6 inbred mouse orthotopic transplanted prostate cancer model and observe the biological characteristics of the growth and metastasis of prostate cancer in vivo. Methods The microscopic surgical technique was used to implant the mouse prostate cancer mass with the intact tissue structure and subcutaneously transplanted into the inbred mice of C57BL6 inoculated between two ventral leaves of the prostate of C57BL6 inbred mice. Tumor metastasis and survival of mice. Results After C57BL6 was treated with orthotopic transplantation of RM-1 tumor, the animals showed general state failure and significant weight loss on the 15th to 20th day. The lower abdomen had palpable mass, all of which were occupied by the tumor, and the prostate tissue could not be seen. In situ model group generally see the cancer tissue extensive infiltration around the pelvic organs, including lymph nodes (16/16), pulmonary (8/16) metastasis. The survival time of tumor-bearing mice in both models was shorter than that in subcutaneous model group (17.0 ± 3.5) days (33.0 ± 5.6) d, the difference was significant. Conclusion This model can simulate the natural growth of mouse prostate cancer in vivo and is suitable for observing the biological characteristics of prostate cancer in vivo.