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用4个粳型水稻品种为材料,研究了30keV下4种剂量N~+离子注入和2种浓度叠氮化钠单独和复合处理的生理效应和诱变效应。结果表明,与单独处理相比,复合处理的生理损伤加剧,M_2代叶绿素突变频率增高,提高了诱变效率。4.0×10~(16)N~+/cm~2和6.0×10~(16)N~+/cm~2离子注入复以2.0mM叠氮化钠处理,以及4.0×10~(16)N~+/cm~2和8.0×10~(16)N~+/cm~2离子注入复以1.0mM叠氮化钠处理的叶绿素突变频率依次为2.96％,3.16％,1.24％和1.87％(M_2苗计),比对应剂量下两种诱变剂单独处理的叶绿素突变频率之和1.89％,2.22％,1.23％和1.39％要高。
Using four japonica rice cultivars as materials, physiological and mutagenic effects of four doses of N + ions at 30 keV and two concentrations of sodium azide were studied. The results showed that compared with the single treatment, the physiological damage of the composite treatment was aggravated, the frequency of chlorophyll mutation in M_2 was increased, and the mutagenesis efficiency was improved. 4.0 × 10 ~ (16) N ~ + / cm ~ 2 and 6.0 × 10 ~ (16) N ~ + / cm ~ 2 were implanted with 2.0mM sodium azide and 4.0 × 10 ~ (16) N The frequency of chlorophyll mutation of 1.0mM sodium azide treated with ~ + / cm ~ 2 and 8.0 × 10 ~ (16) N ~ + / cm ~ 2 ions were 2.96%, 3.16%, 1.24% and 1.87% M_2 seedling meter), which was 1.89% and 2.22%, 1.23% and 1.39% higher than the sum of the chlorophyll mutation frequencies of the two mutagens alone.