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用罗丹明－鬼笔环肽（ｒｈｏｄａｍｉｎｅ－ｐｈａｌｌｏｉｄｉｎ）显示微丝（ｍｉｃｒｏｆｉｌａｍｅｎｔｓ，ＭＦ）的荧光染色法对Ｇ期小鼠Ｃ３Ｈ１０Ｔ１／２成纤维细胞向Ｓ期过渡过程中，ＭＦ结构的变化及ＭＦ重组对ＤＮＡ合成的影响进行了研究，Ｇ期细胞在血清刺激１ｈ后ＭＦ解聚，３ｈ后重新组装，并恢复原来的分布。我们发现细胞松弛素Ｂ（ｃｙｔｏｃｈａｌａｓｉｎＢ，ＣＢ）使ＭＦ解聚并与蛋白激酶Ｃ（ｐｒｏｔｅｉｎｋｉｎａｓｅＣ，ＰＫＣ）激活剂──佛波酯（ｐｈｏｒｂｏｌｅｓｔｅｒ，ＴＰＡ）同样可促进Ｇ期细胞提前进入Ｓ期，促进ＤＮＡ合成，但ＴＰＡ和ＣＢ的刺激作用必需依赖于血清的存在，而ＰＫＣ抑制剂Ｈ７则阻断Ｇ期细胞进入Ｓ期，ＭＦ稳定剂ｐｈａｌｌｏｉｄｉｎ对ＤＮＡ合成具有一定的抑制作用，实验结果表明，Ｇ期细胞向Ｓ期过渡的早期阶段存在ＭＦ重组过程，ＣＢ促使Ｇ细胞ＭＦ解聚并可促进ＤＮＡ合成，提示ＭＦ重组是Ｇ期细胞向Ｓ期过渡的一个重要的早期事件。
Fluorescent staining of microfilaments (MF) with rhodamine-phalloidin showed that the MF structure was changed during the transition from C3H10T1 / 2 fibroblasts to S phase in group G mice and MF reorganization The effects of DNA synthesis were studied. G cells were depolymerized after 1 h of serum stimulation and reassembled after 3 h, and the original distribution was restored. We found that cytochalasin B (CB) depolymerizes MF and activates G-phase cells into S phase as well as activator of protein kinase C (PKC), phorbol ester (TPA), and promotes DNA However, the stimulation of TPA and CB must depend on the presence of serum, whereas the PKC inhibitor H7 blocked the G phase cells entering the S phase. The MF stabilizer phalloidin inhibited the DNA synthesis to a certain extent. The experimental results showed that G phase MF recombination occurs in the early stage of cell-to-S phase transition, CB promotes the decontamination of G cells and promotes DNA synthesis, suggesting that MF recombination is an important early event in the transition from G cells to S phase.