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目的了解丙型肝炎病毒感染的不同人群血清 HCV RNA 水平,比较定性和定量 PCR 结果,探讨 HCV RNA 含量与血清ALT 的相关性.方法采用荧光定量 PCR 和逆转录巢式定性 PCR 同时检测136例丙型肝炎病毒感染者血清 HCV RNA,并测定定量 PCR(+)者血清 ALT.用相关系数分析 HCV RNA 含量与血清ALT 的关系.结果定性 PCR 阳性率为80.88%,定量 PCR 阳性率为77.94%.两者相对符合率为94.12%.定量 PCR(+)血清(106例)HCV RNA 含量在10~(7.04)～10~(10.96) 拷贝·L~(-1).无症状献血员 HCV RNA 含量显著低于慢性丙型肝炎、肝硬变和肝细胞癌患者(P<0.05),肝细胞癌患者血清 ALT 水平显著低于慢性丙型肝炎(P<0.05).HCV RNA 滴度与血清 ALT 呈正相关(r=0.61,P<0.01).结论定性和定量检测结果有很好的一致性.病毒复制水平上升在肝损伤和肝病进展中可能起重要作用.
Objective To understand the serum levels of HCV RNA in different populations infected with hepatitis C virus (HCV) and compare the results of qualitative and quantitative PCR to explore the relationship between the content of HCV RNA and serum ALT.Methods Fluorescence quantitative PCR and reverse transcriptase nested PCR were used to detect 136 HCV The correlation between HCV RNA and serum ALT was analyzed by correlation coefficient.Results The positive rate of qualitative PCR was 80.88%, and the positive rate of quantitative PCR was 77.94%. The relative coincidence rate was 94.12% .The HCV RNA content in 106 cases of quantitative PCR (+) serum was between 10 (7.04) and 10 (10.96) copies · L -1, the content of HCV RNA in asymptomatic blood donors (P <0.05), serum ALT levels in patients with hepatocellular carcinoma were significantly lower than those in patients with chronic hepatitis C (P <0.05), HCV RNA titer was positively correlated with serum ALT (R = 0.61, P <0.01) .Conclusion The qualitative and quantitative detection results are in good agreement.The rising of viral replication may play an important role in the progression of liver injury and liver disease.