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目的:观察转染了质粒PCI-NEO-SNCG后的前列腺癌激素非依赖性细胞系PC-3细胞对抗肿瘤药物顺铂(DDP)、5-氟尿嘧啶(5-FU)、阿霉素(ADM)、长春新碱(VCR)、紫杉醇(TAX)的敏感性,探讨SNCG的表达对各种抗肿瘤药物作用效果的影响。方法:转染质粒PCI-NEO和PCI-NEO-SNCG至PC-3细胞,采用RT-PCR法检测PC-3细胞中SNCG的表达;MTT法检测各抗肿瘤药物对转染后PC-3细胞的抑制作用;流式细胞术分析转染细胞经TAX作用后的细胞周期及凋亡。结果:5种抗肿瘤药物对转染了空载体PCI-NEO质粒及PCI-NEO-SNCG质粒细胞的生长抑制作用均存在时间依赖性;转染PCI-NEO的PC-3细胞组与转染PCI-NEO-SNCG的PC-3细胞组中各种抗肿瘤药物的抑制效果比较显示,DDP、5-FU、ADM、VCR的抑制效果两组间没有显著性差异(P>0.05),而TAX对转染PCI-NEO-SNCG的细胞的抑制率较转染PCI-NEO的细胞显著降低(P<0.01);经TAX处理48 h后,在转染PCI-NEO质粒的细胞中,停留在G2-M期的细胞比例显著高于转染PCI-NEO-SNCG质粒的细胞(P<0.01),而停留在G0-G1期及S期的细胞比例,在转染PCI-NEO质粒的细胞中显著低于转染PCI-NEO-SNCG质粒的细胞(P<0.01);在转染PCI-NEO质粒的细胞中Caspase-3的表达显著高于转染PCI-NEO-SNCG质粒的细胞(P<0.01)。结论:TAX对转染了SNCG基因的PC-3细胞中的生长抑制作用明显降低,提示SNCG的表达可抑制TAX的作用效果,这一发现可为前列腺癌的个体化治疗提供理论依据和指导。
OBJECTIVE: To observe the effects of anticancer drugs cisplatin (DDP), 5-fluorouracil (5-FU), doxorubicin (ADM) and prostate cancer cell line PC- , Vincristine (VCR) and paclitaxel (TAX), and to explore the effect of SNCG expression on the effect of various anti-tumor drugs. Methods: The expression of SNCG in PC-3 cells was detected by RT-PCR and the expression of SNCG in PC-3 cells was transfected with plasmids of PCI-NEO and PCI-NEO-SNCG. The expression of SNCG in PC-3 cells was detected by MTT assay. The effect of TAX on the cell cycle and apoptosis of transfected cells was analyzed by flow cytometry. RESULTS: The antitumor agents had a time-dependent effect on the growth inhibition of plasmid pcDNA-NEO-transfected plasmid and PCI-NEO-SNCG plasmid transfected PC-3 cells transfected with PCI-NEO The inhibitory effect of various anti-tumor drugs in PC-3 cells treated with -NEO-SNCG showed that the inhibitory effect of DDP, 5-FU, ADM and VCR was not significantly different between the two groups (P> 0.05) The inhibition rate of cells transfected with PCI-NEO-SNCG was significantly lower than that of cells transfected with PCI-NEO (P <0.01). After TAX treatment for 48 h, the cells transfected with PCI-NEO plasmid remained in G2- The proportion of cells in M phase was significantly higher than that of the cells transfected with PCI-NEO-SNCG plasmid (P <0.01), but the proportion of cells in G0-G1 phase and S phase was significantly lower in cells transfected with PCI-NEO plasmid The expression of Caspase-3 in PCI-NEO-SNP transfected cells was significantly higher than that in PCI-NEO-SNCG transfected cells (P <0.01) . CONCLUSION: The inhibitory effect of TAX on the growth of PC-3 cells transfected with SNCG gene is significantly reduced, suggesting that the expression of SNCG can inhibit the effect of TAX. This finding may provide a theoretical basis and guidance for the individualized treatment of prostate cancer.