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[目的]研究二氧化氯对大肠杆菌F2噬菌体消毒效果及将大肠杆菌F2噬菌体作为评价消毒效果的指示微生物的可行性进行评价,同时观察残留二氧化氯浓度衰减速度。[方法]采用悬液定量杀菌试验,观察大肠杆菌F2噬菌体对常用化学消毒剂二氧化氯的抵抗力。[结果]二氧化氯投加量为1 mg/L时,作用10 min,对大肠杆菌F2噬菌体的杀灭对数值>4.0;作用30 min,染菌水样中残留二氧化氯浓度分别降解为0.35 mg/L;二氧化氯投加量为2 mg/L时,作用3 min,对大肠杆菌F2噬菌体的杀灭对数值>4.0;作用30 min,染菌水样中残留二氧化氯浓度分别降解为0.68 mg/L。[结论]大肠杆菌F2噬菌体对二氧化氯消毒剂,在常规剂量作用下,可有效灭活大肠杆菌F2噬菌体。因此选择大肠杆菌F2噬菌体作为病毒消毒试验的指示微生物,可明显缩短病毒灭活效果评价周期。二氧化氯衰减速度随浓度和时间的变化而加快。
[Objective] The research aimed to study the disinfection effect of chlorine dioxide on phage of Escherichia coli F2 and evaluate the feasibility of using Escherichia coli F2 bacteriophage as indicator microbes to evaluate the disinfection effect. Meanwhile, the decay rate of residual chlorine dioxide concentration was observed. [Method] The suspension quantitative germicidal test was used to observe the resistance of E. coli F2 bacteriophage to the commonly used chemical disinfectant chlorine dioxide. [Result] When the dosage of chlorine dioxide was 1 mg / L, the killing logarithm of Escherichia coli F2 bacteriophage was> 4.0 for 10 min, and the residual chlorine dioxide concentration in the contaminated water samples degraded to 0.35 mg / L; when the dosage of chlorine dioxide was 2 mg / L, the killing logarithm of E.coli F2 bacteriophage was> 4.0; the concentration of residual chlorine dioxide Degradation was 0.68 mg / L. [Conclusion] The F2 bacteriophage of E. coli can effectively inactivate E. coli F2 bacteriophage under the normal dose of chlorine dioxide disinfectant. Therefore, the choice of E. coli F2 bacteriophage as a virus disinfection test indicator microorganisms, can significantly reduce the cycle of evaluation of virus inactivation. Decay rate of chlorine dioxide with the concentration and time to speed up.