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目的研究三七总皂苷对H2O2致大鼠脑微血管内皮细胞损伤产生保护作用的物质基础。方法分离并培养大鼠脑微血管内皮细胞(RBMEC),以MTT和LDH的释放为指标,观察三七总皂苷(TPNS)、三七皂苷R1、人参皂苷Rg1、Rd、Re和Rb1对RBMEC损伤的保护作用。结果500μmol·L-1的H2O2对原代培养的RBMEC产生明显的损伤。20～200mg·L-1的TPNS,16·0μmol·L-1的R1,37·5～75·0μmol·L-1的Rg1,8·0～16·0μmol·L-1的Rd及5·4～54·0μmol·L-1的Rb1对H2O2致RBMEC的损伤具有明显的保护作用(P<0·05或P<0·01),Re则无此作用。结论TPNS对H2O2所致原代培养的RBMEC损伤具有明显的保护作用,产生这一作用的主要物质基础可能为Rb1、Rg1和Rd。
Objective To study the material basis of panax notoginseng saponins on the protection of rat brain microvascular endothelial cells induced by H2O2. METHODS Rat brain microvascular endothelial cells (RBMEC) were isolated and cultured. MTT and LDH release were used as indicators to observe the effects of Panax notoginseng saponins (TPNS), notoginsenoside R1, ginsenosides Rg1, Rd, Re, and Rb1 on RBMEC damage. Protective effects. Results 500μmol·L-1 H2O2 produced significant damage to primary cultured RBMECs. 20 to 200 mg·L-1 TPNS, 16.0 μmol·L-1 R1, 37·5 to 75.0 μmol·L-1 Rg1, 8·0 to 16·0 μmol·L-1 Rd and 5· Rb1 of 4～54·0μmol·L-1 had obvious protective effect on H2O2-induced injury to RBMEC (P<0.05 or P<0.01), while Re had no such effect. Conclusion TPNS has obvious protective effect on primary cultured RBMECs induced by H2O2. The main material basis for this effect may be Rb1, Rg1 and Rd.