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目的研究血管内皮细胞生长因子（ＶＥＧＦ）与乙型肝炎（乙肝）分级（Ｇ）和分期（Ｓ）的关系。方法２００ 例乙肝活检肝标本用ＶＥＧＦ单克隆抗体进行免疫组化（Ｓ－Ｐ法）染色。结果ＶＥＧＦ在肝组织表达有胞浆型、膜窦型及窦内皮细胞型；ＶＥＧＦ主要由肝细胞分泌，其次在肝窦内皮细胞、贮脂细胞、成肌纤维细胞及单型核细胞也示阳性。无明显病变的血管内皮细胞及肝细胞ＶＥＧＦ染色阴性。提示随分级增高ＴＥＧＦ表达增强，其间有极显著性差异（Ｐ＜０．０１）；而Ｓ低分值者ＶＥＧＦ多示阳性表达，Ｓ较高分值者其多为强阳性表达，两者有明显不同（Ｐ＜０． ０１）。结论 ＶＥＧＦ表达与乙肝 Ｇ和 Ｓ呈正相关。
Objective To study the relationship between vascular endothelial growth factor (VEGF) and hepatitis B (G) and staging (S). Methods 200 cases of liver biopsy specimens were stained with VEGF monoclonal antibody by immunohistochemistry (S-P method). Results VEGF expressed cytoplasm, membrane type and sinus endothelial cell type in liver tissue. VEGF was mainly secreted by hepatocytes, followed by sinusoidal endothelial cells, fat storage cells, myofibroblasts and mononuclear cells. Vascular endothelial cells and hepatocytes without obvious pathological changes were negative for VEGF staining. The results showed that the expression of TEGF increased with the increase of grade, with a significant difference (P <0.01). However, the expression of VEGF in S low score group showed a strong positive expression, Significantly different (P <0.01). Conclusion The expression of VEGF is positively correlated with G and S of hepatitis B virus.