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作者以应激反应基因启动子P_htrA代替携破伤风毒素C片段的质粒pTETnir15中的启动子P_nirB,构建了不同于PTETnir15的质粒pTEThtrAl,将其导入鼠伤寒杆菌BRD509株（SL1344 aroAaroD）,获得含pTEThtrAl的BRD937株。将以启动子控制C片段产量的BRD937（BRD509/PTETh-trAl）、BRD847（BRD509/pTETnir15）或亲本株BRD509分别口饲小鼠,或用吸附于氢氧化铝凝胶的纯化C片段皮下免疫小鼠,用ELISA法检测各组小鼠的抗C片段抗体。
The authors constructed a pTEThtrA1 different from PTETnir15 by introducing P_htrA, a promoter of stress response gene, instead of P_nirB, a promoter of plasmid pTETnir15 carrying the C fragment of Haemophilus toxin, and introduced it into Salmonella typhimurium BRD509 (SL1344 aroAaroD) to obtain pTEThtrAl Of BRD937 strain. Mice were drenched with BRD937 (BRD509 / PTETh-trA1), BRD847 (BRD509 / pTETnir15) or parent strain BRD509 with promoter-controlled C-fragment production, respectively, or subcutaneously immunized with a purified C fragment adsorbed to aluminum hydroxide gel The mice were tested for anti-C fragment antibodies by ELISA.