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目的 探讨bcl-2、bax及肝细胞凋亡在丁型肝炎发病机理中的作用。方法 采用免疫组织化学单、双标记染色和TUNEL技术,检测77例丁型肝炎患者肝组织HDAg、bcl-2、bax及肝细胞凋亡表达,以67例乙型肝炎作对照。结果 bcl-2和bax均以肝细胞浆表达为主。HDAg以肝细胞核表达为主。HDAg和Bax与凋亡细胞表达及分布有相关性,三成分在各型肝炎中的表达强度差异有显著意义(P<0.05)。结论 HDAg、bax和肝细胞凋亡表达强度和阳性细胞分布均与肝组织炎症活动及病理损害程度相关,HDV感染可诱导肝细胞表达bax,增强肝细胞凋亡,这一机制在丁型肝炎发病机理中可能有一定重要作用。
Objective To investigate the role of bcl-2, bax and hepatocyte apoptosis in the pathogenesis of hepatitis type. Methods The expressions of HDAg, bcl-2, bax and hepatocyte apoptosis in hepatic tissue of 77 patients with hepatitis D were detected by immunohistochemical single and double labeling staining and TUNEL technique, 67 cases of hepatitis B were used as control. Results Both bcl-2 and bax were mainly expressed in liver cytoplasm. HDAg is mainly expressed in hepatocytes. HDAg and Bax were correlated with the expression and distribution of apoptotic cells. There was significant difference in the expression intensity of three components among various types of hepatitis (P <0.05). Conclusions The expressions of HDAg, bax and hepatocyte apoptosis and the distribution of positive cells are correlated with the degree of inflammatory activity and pathological damage in liver tissue. HDV infection can induce the expression of bax in hepatocytes and enhance the apoptosis of hepatocytes. Mechanism may have some important role.