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[目的]研究不同植物激素及浓度对羊草种子萌发和幼苗生长的影响。[方法]将GA3和NAA用少量的乙醇助溶,用蒸馏水分别配成浓度为100、300和600μg/g的溶液;将6-BA和2,4-D分别用少量的盐酸和乙醇助溶后,用蒸馏水配成浓度为25、50和75μg/g的溶液。然后,将消毒的羊草种子放在直径为11cm铺有单层滤纸的玻璃培养皿内,每种处理3次重复,每个重复100粒。发芽温度16/24℃,12/12h黑暗和光照,光照强度为54μmol/(m2·s),实验时间为35d,观察并记录种子的萌发情况。[结果]植物激素GA3和6-BA对羊草种子发芽率的影响均表现为低浓度促进,高浓度抑制的趋势。100、300μg/g的GA3或25μg/g6-BA对羊草种子的最终发芽率表现为促进作用,总体效果为GA3>6-BA;而当GA3浓度为600μg/g或6-BA浓度大于50μg/g时,羊草种子的发芽率均表现为不同程度的抑制。任意浓度的NAA和2,4-D对羊草种子萌发抑制作用比较大。在种子萌发初期,NAA对羊草种子萌发均表现为抑制作用;第10～35天时,低浓度NAA和2,4-D对羊草种子萌发略有促进,而高浓度则显著抑制。100、300和600μg/gNAA处理的羊草种子最终发芽率均低于对照,超过300μg/g处理的种子发芽率显著受到抑制,600μg/gNAA处理的发芽率仅为5.2%,比对照降低了30%左右。25、50μg/g2,4-D处理的羊草种子最终发芽率均低于对照,75μg/g处理的羊草种子发芽率受到显著抑制,发芽率仅为4.3%,比对照降低了30%左右。此外,NAA和2,4-D对羊草种子播种后的初始萌发时间影响很大,对照为3d,100和300μg/gNAA处理的种子为5d,而600μg/gNAA处理的种子为30d。25、50μg/g2,4-D处理的种子为5d,而75μg/g处理的为24d。GA3和6-BA处理均能显著促进羊草幼苗的生长,总体效果为GA3>6-BA,NAA和2,4-D对幼苗生长则表现为不同程度的抑制作用。整个过程中,幼苗生长速度缓慢。幼苗开始略有生长,后期则生长缓慢,直至死亡。[结论]不同植物激素对羊草种子萌发和幼苗生长产生不同的影响。
[Objective] The research aimed to study the effects of different plant hormones and concentrations on the seed germination and seedling growth of Leymus chinensis. [Method] GA3 and NAA were solubilized with a small amount of ethanol, and the solutions were prepared into 100, 300 and 600 μg / g respectively with distilled water. The 6-BA and 2,4-D were dissolved in a small amount of hydrochloric acid and ethanol respectively The solution was then made up to 25, 50 and 75 μg / g with distilled water. The sterilized Leymus chinensis seeds were then placed in glass dishes of 11 cm in diameter and covered with a single layer of filter paper, three replicates for each treatment, each of 100 replicates. Germination temperature 16/24 ℃, 12 / 12h dark and light, light intensity 54μmol / (m2 · s), the experimental time 35d, observe and record the seed germination. [Result] The effects of plant hormones GA3 and 6-BA on the seed germination rate of Leymus chinensis showed the tendency of low concentration and high concentration. The final germination rate of 100, 300μg / g GA3 or 25μg / g 6-BA on the seeds of Leymus chinensis played a promoting role, with the overall effect of GA3> 6-BA. When GA3 concentration was 600μg / g or 6-BA concentration was more than 50μg / g, the germination rate of Leymus chinensis seeds showed different degrees of inhibition. At any concentration of NAA and 2,4-D on the germination of Leymus chinensis seed germination relatively large. At the early stage of seed germination, NAA showed inhibition on the seed germination of Leymus chinensis. At the first to 35th day, NAA and 2,4-D at low concentration promoted the germination of Leymus chinensis seeds slightly, but inhibited significantly at high concentration. The final germination rate of L. chinense seeds treated with 100, 300 and 600 μg / g NAA was lower than that of the control, the germination rate of the seeds treated with 300 μg / g was significantly inhibited, and the germination rate was only 5.2% %about. The final germination rate of Leymus chinensis seeds treated with 25 and 50μg / g 2,4-D was lower than that of the control, and the germination rate of seeds treated with 75μg / g Leymus chinensis was significantly inhibited, the germination rate was only 4.3%, about 30% lower than that of the control . In addition, NAA and 2,4-D had a significant effect on the initial germination time after sowing of Leymus chinensis seeds. The control treatments were 3d, 100 and 300 μg / g NAA for 5 days, and 600 μg / g NAA-treated seeds for 30 days. Seeds at 25, 50 μg / g 2,4-D were 5 days and 24 days at 75 μg / g. Both GA3 and 6-BA could significantly promote the growth of Leymus chinensis seedlings. The overall effect was GA3> 6-BA. NAA and 2,4-D showed different degrees of inhibition on seedling growth. Throughout the process, seedlings grow slowly. Seedlings begin to grow slightly, while later grow slowly until they die. [Conclusion] Different plant hormones had different effects on the seed germination and seedling growth of Leymus chinensis.