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中性多形核白细胞(PMN)在体外经氟康唑(FCZ)预孵育后,其杀菌活性增强。本实验旨在确定FCZ诱导PMN功能增强中细胞因子的作用。分离12位供者白细胞,经处理获得的PMN浓度范围为3×10~6/ml～1×10~7/ml,纯度和活力超过90％。试验菌株为大肠埃希菌标准菌株(ATCC 25922),在37℃下经磷酸盐缓冲液(PBS)或FCZ预孵育过夜,细菌浓度在对数生长期为10~9/ml。组1(细菌组,即对照组):1ml细菌液、0.2ml PBS、1ml培养基和0.2ml病人血
Neutrophilic polymorphonuclear leukocytes (PMNs) showed enhanced bactericidal activity after preincubation with fluconazole (FCZ) in vitro. The aim of this experiment was to determine the role of cytokines in FCZ-induced enhancement of PMN function. Twelve donor leukocytes were isolated. The PMN concentration ranged from 3 × 10 ~ 6 / ml to 1 × 10 ~ 7 / ml and the purity and viability exceeded 90%. The test strain was an Escherichia coli standard strain (ATCC 25922) and was pre-incubated overnight at 37 ° C with phosphate buffered saline (PBS) or FCZ at a bacterial concentration of 10-9 / ml in logarithmic growth phase. Group 1 (bacteria group, control group): 1 ml of bacterial liquid, 0.2 ml of PBS, 1 ml of culture medium and 0.2 ml of patient’s blood