论文部分内容阅读
目的探讨福建地区男性不育与精液参数和精子DNA完整性关系。方法对5680例男性不育患者和3200例健康体检者进行精液参数和精子DNA完整性检测。采用SQA-V全自动精子分析仪进行精液常规分析,精子形态采用改良巴氏染色法,精子DNA完整性检测采用精子染色质扩散试验(SCD),用DNA断裂指数(DFI)表示。结果不育患者精子精子密度、活动率、精子形态和DFI与对照组比较差异有统计学意义(P<0.05);两组的精液量、精液p H值差异无统计学意义(P>0.05)。结论精子密度、活动率、精子形态和DFI的异常是引起福建地区男性患者不育的最主要原因。
Objective To investigate the relationship between male infertility and semen parameters and sperm DNA integrity in Fujian Province. Methods The sperm parameters and sperm DNA integrity of 5680 male infertility patients and 3200 healthy subjects were tested. Sperm routine analysis was performed with SQA-V automatic sperm analyzer. Sperm morphology was modified by Papanicolaou staining. Sperm DNA integrity was detected by sperm chromatin diffusion test (SCD) and DNA fragmentation index (DFI). Results The sperm motility, sperm motility, morphology and DFI in infertile patients were significantly different from those in the control group (P <0.05). There was no significant difference in semen volume and semen p value between the two groups (P> 0.05) . Conclusion Sperm density, activity rate, sperm morphology and DFI abnormalities are the most common causes of male infertility in Fujian area.