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目的对无锡市人群、动物及节肢类虫媒生物新布尼亚病毒感染情况,分析感染谱、流行病学特点及影响因素,为控制疫情提供依据。方法采用统一的诊断标准调查病例,双抗原夹心酶联免疫吸附测定法(enzymelinked immunosorbent assay,ELISA)法检测血清特异性总抗体,采用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)法进行病原学诊断。结果 2010-2011年,共调查发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)疑似病例66例,疑似病例确诊阳性率为7.58%,确诊病例病死率为60.00%(3/5)。一般人群总抗体阳性率为0.23%(2/866);家畜类动物337只,抗体阳性率为2.08%,阳性率较高的动物为鹅(33.33%)、羊(6.76%)和鸡(0.67%);野生类动物115只,抗体阳性率为1.74%,阳性率较高的动物为鼠(2.13%)。节肢类虫媒生物中未发现核酸检测阳性者。结论初步确认新布尼亚病毒感染谱包括隐性感染和显性感染(包括轻症病例、重症病例和死亡病例);该病可能为人畜共患病,可能宿主动物为鼠、羊和鸡;年龄偏大和生活在丘陵地区可能是SFTS发病的危险因素。
Objective To investigate the infection status of neubrian virus in population, animals and arthropods in Wuxi City, analyze the spectrum of infection, epidemiological characteristics and influencing factors, and provide basis for controlling epidemic situation. Methods Uniform diagnostic criteria were used to investigate the cases. Serum specific total antibodies were detected by enzyme linked immunosorbent assay (ELISA), and the reverse transcription - polymerase chain reaction (RT - PCR) -PCR) method for etiological diagnosis. Results From 2010 to 2011, 66 suspected cases of fever with thrombocytopenia syndrome (SFTS) were investigated. The positive rate of suspected cases was 7.58% and the case fatality rate was 60.00% (3/5). The positive rate of total antibody in the general population was 0.23% (2/866), 337 for domestic animals, the positive rate of antibody was 2.08%, and the positive rate was 33.33%, 6.76% and 0.67 %); 115 wild animals, the positive rate of antibody was 1.74%, the positive rate of animals was rats (2.13%). No detectable nucleic acid was found in arthropod insects. Conclusions Preliminary identification of the new Bunyan virus infection spectrum includes latent infection and dominant infection (including mild cases, severe cases and deaths); the disease may be zoonosis, the possible host animals are mice, sheep and chickens; Older age and living in hilly areas may be risk factors for the onset of SFTS.