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目的研究口服蚯蚓纤溶酶（ＥＦＥ）对纤溶系统和血小板聚集功能的影响。方法正常雄性Ｂｅａｇｌｅ犬，体重６．５～９ｋｇ，经口给蚯蚓纤溶酶，经前肢静脉取血。以Ｓ－２２５１为发色底物，测定血浆纤溶酶活性；５０μｍｏｌ／ＬＡＤＰ为诱导剂测定血小板聚集率。结果（１）单剂量ＥＦＥ４０、８０ｍｇ／ｋｇ后血浆纤溶酶活性升高（Ｐ＜０．０５），８０ｍｇ／ｋｇ组高于４０ｍｇ／ｋｇ组（Ｐ＜０．０５）。连续给药１０ｄ，４０、８０ｍｇ／ｋｇ组均有多个时间点纤溶酶活性升高（Ｐ＜０．０５），但两组间无差异。（２）单剂量ＥＦＥ４０ｍｇ／ｋｇ，或经口给阿司匹林８ｍｇ／ｋｇ后３ｈ，血小板聚集均被抑制（Ｐ＜０．０５）；而在单剂量ＥＦＥ８０ｍｇ／ｋｇ或经口给淀粉后３ｈ，血小板聚集功能均无变化。结论ＥＦＥ经口给药可提高血浆纤溶酶活性，并在一定剂量下可抑制血小板的聚集。
Objective To study the effect of oral earthworm fibrinolytic enzyme (EFE) on fibrinolysis and platelet aggregation. Methods Normal male Beagle dogs, body weight 6.5 ~ 9kg, earthworm fibrinolytic enzyme by mouth, forelimb vein blood. The plasmin activity was measured by S-2251 as chromogenic substrate. Platelet aggregation was measured by using 50μmol / L ADP as an inducer. Results (1) After the single dose of EFE40,80 mg / kg, plasmin activity increased (P <0.05), 80 mg / kg group was higher than 40 mg / kg group (P <0.05). Continuous administration of 10d, 40,80 mg / kg group were multiple fibrinolytic activity increased (P <0.05), but no difference between the two groups. (2) Platelet aggregation was inhibited by a single dose of EFE40mg / kg, or 3h after oral administration of aspirin at 8mg / kg (P <0.05). However, platelets were aggregated at a single dose of EFE80mg / kg or 3h after oral administration of starch No change in function. Conclusion EFE oral administration can increase plasma plasminogen activity, and in a certain dose can inhibit platelet aggregation.