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探讨驱虫斑鸠菊(VW)注射液治疗白癜风的作用机制。进行以下实验探讨VW对小鼠免疫功能的影响:淋巴细胞转化试验测定小鼠脾T、B细胞增殖活性;脾细胞介导羊红细胞定量溶血分光光度法测定B细胞生成抗体活性,流式细胞法测定B细胞上CD19表达活性;迟发型超敏反应(DTH)试验测定T细胞活性、眼3H演-TdR掺入法测定T细胞分泌IL-2活性等。用酶学方法研究VW对小鼠体内酪氨酸酶的作用。用半定量逆转录聚合酶链反应技术检测酪氨酸酶基因表达活性。结果表明,VW可以明显抑制小鼠体内T、B细胞的增殖反应(P<0.01);对绵羊红细胞(SRBC)诱导的正常小鼠脾脏抗体形成细胞活性、CD19B细胞亚类表达、小鼠DTH反应和T细胞分泌IL-2活性也具有明显的抑制作用,这些抑制作用与药物浓度有一定的剂量效应关系。VW还可以提高小鼠血清酪氨酸酶活性,增强酪氨酸酶基因的表达。以上结果说明,VW可抑制小鼠免疫功能,可以从转录水平增强酪氨酸酶活性,进而促进黑素合成。
To investigate the mechanism of treatment of vitiligo by vermonia vermonia (VW) injection. The following experiments were conducted to investigate the effect of VW on immune function in mice: Lymphocyte transformation assay was used to determine the activity of splenic T and B cell proliferation; Spleen cells were used to quantify red blood cell quantitative hemolytic spectrophotometry to determine the activity of B cells to generate antibodies. Flow cytometry The activity of CD19 on B cells was measured; the activity of T cells was measured by delayed type hypersensitivity (DTH) test, and the activity of IL-2 secreted by T cells was measured by 3H-TdR incorporation. The effect of VW on tyrosinase in mice was studied enzymatically. Tyrosinase gene expression activity was measured by semiquantitative reverse transcription polymerase chain reaction. The results showed that VW could significantly inhibit the proliferation of T and B cells in mice (P<0.01); the activity of splenic antibody-forming cells, the expression of CD19B subclass, and the DTH response of mice induced by sheep erythrocytes (SRBC) And T cells secrete IL-2 activity also has a significant inhibitory effect, these inhibitory effects and drug concentration have a certain dose-effect relationship. VW can also increase serum tyrosinase activity and increase tyrosinase gene expression in mice. The above results show that VW can inhibit the immune function of mice and can increase tyrosinase activity from the transcriptional level, thereby promoting melanin synthesis.