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锌元素的营养失衡已成为影响人类健康的最重要因素之一,籽粒锌含量的QTL(quantitative trait loci)定位对研究富锌水稻的遗传育种具有重要的意义。以水稻(Oryza sativa L.)亲本奉新红米和明恢100杂交的145个株系的F2群体为实验材料,利用92个SSR(simple sequence repeat)标记对水稻籽粒锌含量进行了QTL定位,共检测到3个QTLs,分别定位于第3、6和11染色体上,对表型变异的贡献率分别为4.97%、12.75%和7.74%。其中位于第3染色体上的分子标记RM186和RM168之间的QZN3对表型变异的贡献率最大,其增效等位基因来自亲本明恢100,表现为部分显性。3个QTLs的联合贡献率为25.46%,具有基因累加效应。该研究结果有利于深入理解水稻锌含量的遗传基础,为锌含量的QTL精细定位、基因克隆和分子标记辅助选择提供依据。
Nutrient imbalance of zinc has become one of the most important factors that affect human health. The mapping of quantitative trait loci (QTL) of grain zinc content is of great significance for the study of genetic breeding of zinc-rich rice. F2 population from 145 lines of new red rice (Oryza sativa L.) and the cross of Minghui100 was used as experimental material, and 92 SSR (simple sequence repeat) markers were used to locate QTLs of rice grain zinc content. Three QTLs were detected and located on chromosomes 3, 6 and 11, respectively. The contribution rates to phenotypic variation were 4.97%, 12.75% and 7.74%, respectively. Among them, the QZN3 molecular marker RM186 and RM168 located on chromosome 3 contributed the most to the phenotypic variation. The allele of QZN3 was from the parent Minghui 100 and showed partial dominant. The combined contribution rate of three QTLs was 25.46%, with gene accumulation effect. The results of this study are helpful to understand the genetic basis of zinc content in rice and provide the basis for fine QTL mapping of zinc content, gene cloning and molecular marker-assisted selection.