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目的:观察联合应用奈达铂和放疗治疗荷Lewis肺癌实体瘤C57BL小鼠的疗效,以明确奈达铂对Lewis瘤的放射增敏作用。方法:建立小鼠Lewis肺癌实体瘤模型后,分对照组、单纯放疗组、奈达铂组及奈达铂联合放疗组。对照组给予腹腔注射生理氯化钠溶液0.1 mL,单纯放疗组荷瘤鼠左前肢肿瘤局部以15GY射线照射,奈达铂组分2个亚组,分别以5和10 mg.kg-1奈达铂腹腔注射,联合放疗组则以5 mg.kg-1奈达铂腹腔注射加荷瘤鼠左前肢肿瘤局部15GY射线照射。然后每隔2 d测量肿瘤的长、短径,比较各组瘤体积、肿瘤生长延缓天数、荷瘤鼠存活数和肺转移率。结果:与对照组比较,5和10 mg.kg-1的单剂量奈达铂都对Lewis实体瘤生长有抑制作用(P<0.001);联合放疗组局部肿瘤生长受到明显抑制,抑瘤率为76.88%,明显高于单纯放疗组(57.21%,P<0.001)和奈达铂组(23.57%,P<0.001);联合放疗组肿瘤生长延缓天数(瘤体积1.1~2.5 cm3)超过10 d,高于单纯放疗组(6 d,P<0.001)和奈达铂组(4 d,P<0.001);联合放疗组60 d内存活的鼠最多(3个),单纯放疗组为1个,其他两组鼠全部死亡。联合放疗组平均肺转移为0.8个/叶肺,单纯放疗组为2.2个/叶肺,奈达铂组为6.04个/叶肺,对照组为20.2个/叶肺(前三者与对照组比较P<0.001,联合放疗组与单纯放疗组比较P<0.01)。结论:奈达铂可显著地增强小鼠Lewis肺癌细胞放射治疗敏感性,并减少肺转移。
OBJECTIVE: To observe the curative effect of combined use of nedaplatin and radiotherapy on C57BL mice bearing solid Lewis lung carcinoma, in order to clarify the radiosensitization effect of nedaplatin on Lewis tumors. Methods: After establishing Lewis lung cancer solid tumor model in mice, the control group, radiotherapy alone group, nedaplatin group and nedaplatin combined radiotherapy group. The control group was given intraperitoneal injection of physiological sodium chloride solution 0.1 mL, radiotherapy alone tumor group left forelimb tumor local 15GY ray irradiation, nedaplatin component 2 subgroups, respectively, 5 and 10 mg.kg-1 Nida Platinum intraperitoneal injection, combined with radiotherapy group was 5 mg.kg-1 nedaplatin intraperitoneal injection of tumor-bearing mice left forelimb tumor local 15GY ray irradiation. Then every 2 days, the length and the short diameter of the tumor were measured. The tumor volume, the number of days of tumor growth delay, the survival of tumor-bearing mice and lung metastasis were compared. Results: Compared with the control group, 5 and 10 mg.kg-1 single dose of nedaplatin all inhibited the growth of Lewis solid tumors (P <0.001). The combination of radiotherapy local tumor growth was significantly inhibited tumor inhibition rate 76.88%, which was significantly higher than that of radiotherapy alone (57.21%, P <0.001) and nedaplatin group (23.57%, P <0.001). The tumor growth retardation days (tumor volume 1.1 ~ 2.5cm3) (4 d, P <0.001). Compared with the radiotherapy alone group (4 d, P <0.001), the rats in the radiotherapy group had the highest number of surviving mice in 60 d Two groups of mice all died. In the combined radiotherapy group, the average pulmonary metastases were 0.8 lungs / leaf lungs, 2.2 radiotherapy alone groups, 6.04 lungs / leaf lungs in the nedaplatin group and 20.2 lungs / leaf lungs in the control group (the former three were compared with the control group P <0.001, combined radiotherapy group and radiotherapy alone group P <0.01). Conclusion: Nedaplatin significantly enhances the radiosensitivity of Lewis lung carcinoma cells in mice and decreases lung metastasis.