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目的:探讨逍遥散降低雌性兔膝关节软骨细胞凋亡的可能作用机制。方法:40只新西兰兔通过手术造成膝关节失稳型模型,1周后随机分为中药组、西药组、混合组和对照组各10只,驱赶行走4周,分别给药8周。造模后12周末处理动物,免疫组化法检测兔膝关节软骨细胞凋亡线粒体通路相关基因的表达。结果:中药组、西药组和混合组Bcl-2表达均显著高于对照组;Bax、Caspase-3与细胞色素的C表达均显著低于对照组。结论:逍遥散可明显上调Bcl-2表达,下调Bax、Caspase-3与细胞色素的C的表达,可能是逍遥散降低兔膝关节软骨细胞凋亡的机制之一。
Objective: To explore the possible mechanism of Xiao Yao San to reduce the apoptosis of knee joint chondrocytes in female rabbits. Methods: Forty New Zealand rabbits were operated on to create an unstable knee joint model. One week later, 10 rabbits were randomly divided into TCM group, western medicine group, mixed group and control group. Each group was driven for 4 weeks and administered for 8 weeks respectively. Animals were sacrificed at 12 weeks after modeling, and the expression of mitochondrial pathway-related genes in rabbit knee articular cartilage cells was detected by immunohistochemistry. Results: The expressions of Bcl-2 in Chinese medicine group, western medicine group and mixed group were significantly higher than those in control group. The expressions of Bax, Caspase-3 and cytochrome C were significantly lower than those in control group. Conclusion: Xiaoyaosan can obviously up-regulate the expression of Bcl-2, and down-regulate the expressions of Bax, Caspase-3 and cytochrome C, which may be one of the mechanisms that Xiaoyao Powder can reduce the apoptosis of rabbit articular cartilage.