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本文利用生物信息学方法对森林草莓(Fragaria vesca)基因组数据库中MADS-box基因的数量、结构类型、序列特征及染色体定位进行分析。结果表明,获得70个含SRF-TF结构域的森林草莓Fv MADS基因,DNA长度289~14 596 bp,编码66~1 437个氨基酸残基,有21个Fv MADS没有内含子,在7条染色体上呈不均匀分布;68个Fv MADS蛋白序列含有保守基序Motif 1,最佳匹配序列为“RQVTFSKRRNGLLKKAYELSVLCDAEVALIIFSSRGKLYEF”。另外,从栽培品种‘丰香’草莓果实中克隆了Fa MADS1基因,该基因属于MADS-box基因家族,c DNA全长1 167 bp,编码区750 bp,推导编码249个氨基酸,具有MADS结构域和K-box结构域。
In this paper, the number of MADS-box genes in Fragaria vesca genomic database, their structure types, sequence characteristics and chromosome location were analyzed by using bioinformatics methods. The results showed that 70 Fv MADS genes of forest strawberry with SRF-TF domain were obtained. The length of DNA was 289-14 596 bp, encoding 66-1 437 amino acid residues, 21 Fv MADS without introns, The 68 Fv MADS protein sequences contained the motif Motif 1, and the best matching sequence was “RQVTFSKRRNGLLKKAYELSVLCDAEVALIIFSSRGKLYEF ”. In addition, the Fa MADS1 gene was cloned from the cultivated ’Fengxiang’ strawberry fruit. The gene belongs to the MADS-box gene family. The c DNA is 1 167 bp in length and 750 bp in length and deduced to encode a protein of 249 amino acids with the MADS domain And K-box domains.