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目的探讨G细胞异常凋亡对肾阳虚模型大鼠消化功能的影响。方法建立肾阳虚证模型大鼠的基础上,采用TUNEL法、免疫组化法检测胃窦粘膜组织的细胞凋亡、GAS分泌G细胞及其凋亡相关蛋白Caspase-3的表达,并以假手术组作为对照组,参附汤组作为治疗组。结果模型组大鼠胃窦粘膜组织细胞的AI显著升高;胃窦粘膜组织G细胞的平均光密度值显著下降;胃窦粘膜组织G细胞表达凋亡相关基因Caspase-3的百分率显著升高。参附汤组大鼠的各病理指标均明显好转。结论胃窦粘膜组织及G细胞的过度凋亡是肾阳虚影响消化功能的主要机制之一,参附汤可改善此病理改变。
Objective To investigate the effect of abnormal G cell apoptosis on the digestive function in rats with kidney-yang deficiency. Methods On the basis of establishing the model rats with deficiency of kidney-Yang syndrome, the apoptosis of gastric antral mucosa was detected by TUNEL method and the expression of G-cell and its associated protein Caspase-3 were detected by GAS. The operation group as the control group, Shenfu Tang group as the treatment group. Results The AI of antral mucosa in model group was significantly increased. The average optical density of G cells in antral mucosa significantly decreased. The percentage of apoptotic gene Caspase-3 in gastric antral mucosa was significantly increased. Shenfu Tang rats in each pathological indicators were significantly improved. Conclusion Apoptosis of gastric antral mucosa and G cells is one of the main mechanisms that affect the digestive function of kidney yang deficiency. Shenfu decoction can ameliorate this pathological change.