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本实验采用大鼠40只,雌雄各半,随机分为海平对照、缺氧即刻、缺氧20min和缺氧60min 4组。缺氧在低压舱中进行,模拟海拔高度为8000m。用放免和HPLC法分别测定了4组动物脑组织中腺苷、AMP和血管活性肠肽(VIP)含量的变化。结果表明,3个缺氧组腺苷水平明显高于海平对照组(66.98±4.52μg/g),以缺氧即刻组为最高(108.15±10.59μg/g,P<0.01)。脑组织中VIP含量,海平对照组为46.15±3.83pmol/g,缺氧即刻略有下降,以后逐渐上升,至缺氧60min时达67.75±3.11pmol/g,明显高于对照组(P<0.05)。推测二者与缺氧时脑血流增加有关。
In this experiment, 40 rats were used, randomly divided into sea level control, hypoxic exposure, hypoxia 20min and hypoxia 60min 4 groups. Hypoxia was carried out in a low pressure chamber at a simulated altitude of 8000 m. The contents of adenosine, AMP and vasoactive intestinal peptide (VIP) in the brains of four groups were determined by radioimmunoassay and HPLC respectively. The results showed that the levels of adenosine in the three hypoxia groups were significantly higher than those in the sea level control group (66.98 ± 4.52μg / g), and those in the hypoxia group were the highest (108.15 ± 10.59μg / g, P <0.01). The content of VIP in the brain tissue was 46.15 ± 3.83 pmol / g in the sea level control group, the hypoxia decreased slightly and then gradually increased to 67.75 ± 3.11 pmol / g at 60 min of hypoxia, which was significantly higher than that of the control group (P <0.05 ). Presumably both are associated with increased cerebral blood flow during hypoxia.