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目的探讨鼻咽癌动态对比增强磁共振扫描(DCE-MRI)参数与乏氧诱导因子-1α(HIF-1α)表达的相关性,并分析两者与鼻咽癌临床病理特征之间的关系。方法对62例鼻咽癌患者行DCE-MRI扫描,获取感兴趣区域的DCE-MRI参数,并在标记处行鼻咽镜活检,用免疫组化方法检测鼻咽癌组织中HIF-1α的表达水平,另检测20例鼻咽慢性炎性组织的HIF-1α表达用于对照;观察DCE-MRI曲线特征,并对DCE-MRI参数和HIF-1α表达情况以及两者与患者临床病理特征的相关性进行分析。结果鼻咽癌DCE-MRI参数中,增强剂流入肿瘤时间(T1 on set)和最大上升斜率(MS)在不同患者性别、年龄间差异无统计学意义(P>0.05),在T分期、N分期和临床分期间差异有统计学意义(P<0.05)。鼻咽癌组织和鼻咽慢性炎性组织中HIF-1α蛋白的阳性表达率分别为79.0%和0,两组间比较差异有统计学意义(P<0.05);HIF-1α表达在不同患者年龄、N分期间差异有统计学意义(P<0.05),而在不同患者性别、T分期和临床分期间差异无统计学意义(P>0.05)。HIF-1α的阳性表达与T1 on set和曲线下面积有明显相关(回归系数分别为-1.817和0.014,P<0.05),而与MS和达峰时间无明显相关性(回归系数分别为0.005和1.009,P>0.05)。结论在鼻咽癌中,DCE-MRI相关参数与癌组织HIF-1α蛋白的阳性表达密切相关,且与鼻咽癌的临床病理特征密切相关。DCE-MRI技术的应用有助于评估鼻咽癌组织的乏氧状况,并在鼻咽癌的辅助诊断、临床分期及预后判断中有一定的临床价值。
Objective To investigate the relationship between the parameters of dynamic contrast-enhanced magnetic resonance (DCE-MRI) and hypoxia inducible factor-1α (HIF-1α) expression in nasopharyngeal carcinoma and to analyze the relationship between them and clinicopathological features of nasopharyngeal carcinoma. Methods Sixty-two patients with nasopharyngeal carcinoma underwent DCE-MRI scanning. The parameters of DCE-MRI in the region of interest were obtained. Nasopharyngeal biopsies were taken at the labeled sites. The expression of HIF-1α in nasopharyngeal carcinoma tissues was detected by immunohistochemistry , And the other 20 cases of nasopharyngeal chronic inflammatory tissue were detected for the expression of HIF-1α. The DCE-MRI curve was observed and the correlation between DCE-MRI parameters and the expression of HIF-1α and the clinicopathological features were analyzed Sex analysis. Results In the DCE-MRI parameters of nasopharyngeal carcinoma, the T1 on set and the maximum rising gradient (MS) were not significantly different in different sexes and ages (P> 0.05). In T stage, N Staging and clinical stage differences were statistically significant (P <0.05). The positive rates of HIF-1α protein in nasopharyngeal carcinoma and nasopharyngeal chronic inflammatory tissues were 79.0% and 0 respectively, there was significant difference between the two groups (P <0.05). The expression of HIF-1α in different age groups (P <0.05). There was no significant difference in gender, T stage and clinical stage between different patients (P> 0.05). The positive expression of HIF-1α was significantly correlated with T1 on set and area under the curve (regression coefficients were -1.817 and 0.014, respectively, P <0.05), but not with MS and peak time (regression coefficients were 0.005 and 1.009, P> 0.05). Conclusion In NPC, DCE-MRI-related parameters are closely related to the positive expression of HIF-1α protein in cancerous tissues, which is closely related to the clinicopathological features of NPC. The application of DCE-MRI technique can help to assess the hypoxic condition of nasopharyngeal carcinoma and has some clinical value in the auxiliary diagnosis, clinical stage and prognosis of nasopharyngeal carcinoma.