论文部分内容阅读
目的：黑色素瘤Ｂ１６细胞胞浆ＨＳＰ－抗原肽复合物（ＨＡＣ）的制备、免疫原性的诱导及其抑瘤作用的研究。方法：采用Ｔｒｉｓ－ＨＣＩ提取和Ｓｅｐｈａｃｒｙｌ Ｓ－２００凝胶过滤制备Ｂ１６细胞ＨＡＣ，通过Ｃ５７ＢＬ／６Ｊ小鼠体内诱导特异性ＣＴＬ，再经体内、体外实验检测其抑癌作用。结果：凝胶过滤获得的含６０～９７ｋＤ蛋白的４１，４７和５３管的ＨＡＣ可以降低肿瘤发生率、延长肿瘤出现时间及降低小鼠死亡率。结论：Ｂ１６细胞胞浆中的６０～９７ｋＤ的ＨＡＣ具有免疫原性及抑瘤作用，为制备肿瘤疫苗提供了实验依据。
OBJECTIVE: To study the preparation of the cytosolic HSP-antigen peptide complex (HAC) of B16 melanoma, the induction of immunogenicity and the anti-tumor effect. Methods: The HAC of B16 cells was prepared by Tris-HCI extraction and Sephacryl S-200 gel filtration. The specific CTLs were induced in C57BL / 6J mice and the anti-tumor effects were detected by in vitro and in vivo experiments. Results: HAC with 41, 47 and 53 tubes containing 60-97 kD protein obtained by gel filtration could reduce the incidence of tumor, prolong tumor appearance and reduce the mortality of mice. Conclusion: HAC from 60 to 97kD in the cytoplasm of B16 cells has immunogenicity and antitumor activity, which provides an experimental basis for the preparation of tumor vaccine.