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目的 :研究增殖细胞核抗原 (PCNA )在胆囊良恶性病变组织的表达及意义。方法 :应用 SP免疫组化法检测 35例原发性胆囊癌 ,10例胆囊腺瘤及腺瘤样病变组织 ,10例慢性胆囊炎中 PCNA表达结果。结果 :PCNA在原发性胆囊癌 ,胆囊腺瘤及腺瘤样病变组织和慢性胆囊炎中的阳性表达指数分别为 43.84%± 18.5 3%、6 .35 %±4.17%、4.5 8%± 3.5 8% ,原发性胆囊癌 PCNA L I明显高于胆囊良性病变组织 (P<0 .0 1)。胆囊癌分化程度越低 ,Nevin分期越高 ,PCNA L I有增高的趋势 (P<0 .0 1)。胆囊癌分化程度与 PCNA L I相关系数为 0 .3719(P=0 .0 2 78)。PCNA L I≤ 43.84%的胆囊癌患者 ,其生存率明显高于 PCNA L I>43.84%者 (P<0 .0 1)。结论 :原发性胆囊癌的发生、发展与细胞异常增殖有关 ,PCNA对胆囊癌的鉴别诊断、判断转移与否及预后有重要价值
Objective: To study the expression and significance of proliferating cell nuclear antigen (PCNA) in benign and malignant lesions of gallbladder. Methods : SP immunohistochemistry was used to detect the expression of PCNA in 35 cases of primary gallbladder carcinoma, 10 cases of gallbladder adenoma and adenomatoid lesions, and 10 cases of chronic cholecystitis. Results: The positive expression indexes of PCNA in primary gallbladder carcinoma, gallbladder adenoma, adenomatoid lesions, and chronic cholecystitis were 43.84% ± 18.53 3%, 6.35% ± 4.17%, and 4.58% ± 3.5 respectively. In 8%, PCNA LI was significantly higher in primary gallbladder carcinoma than in gallbladder benign lesions (P < 0.01). The lower the degree of differentiation of gallbladder carcinoma, the higher the Nevin stage, the tendency of PCNA L I to increase (P < 0.01). The correlation coefficient between gallbladder carcinoma differentiation and PCNA L I was 0.797 (P=0.0 2 78). The survival rate of PCNA L I≤43.84% patients with gallbladder cancer was significantly higher than that of PCNA L I>43.84% (P<0.01). Conclusion: The occurrence and development of primary gallbladder carcinoma is related to the abnormal cell proliferation. PCNA has important value in the differential diagnosis, judgement of metastasis and prognosis of gallbladder carcinoma.