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以罗布麻为试材,采用农杆菌介导法将罗布麻eIF-5A基因导入烟草,分别对正常生长条件和NaHCO_3胁迫下转AveIF-5A基因烟草和非转基因烟草的过氧化氢酶(POD)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量进行测定,以期为罗布麻eIF-5A基因功能研究提供参考。结果表明:正常生长条件下,转AveIF-5A基因烟草和非转基因烟草POD活性和MDA含量差异不显著,转基因植株SOD活性显著高于非转基因植株;在NaHCO_3胁迫下,转AveIF-5A基因烟草POD活性和SOD活性均极显著高于非转基因植株,而MDA含量极显著低于非转基因植株。AveIF-5A基因过量表达可以提高转基因烟草的耐盐能力。
Apocynum venetum was used to induce eIF-5A gene into tobacco by Agrobacterium tumefaciens-mediated transformation. The normal growth conditions and catalase (POD) activity of AveIF-5A transgenic tobacco and non-transgenic tobacco under NaHCO3 stress Activity, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were determined in order to provide a reference for the study of the function of eIF-5A gene in Apocynum venetum. The results showed that there was no significant difference in POD activity and MDA content between wild-type and non-transgenic tobacco under normal growth conditions, while the SOD activity in transgenic plants was significantly higher than that in non-transgenic plants. Under NaHCO_3 stress, the POD activity of AveIF-5A transgenic tobacco Activity and SOD activity were significantly higher than non-transgenic plants, while MDA content was significantly lower than non-transgenic plants. AveIF-5A gene overexpression can improve the salt tolerance of transgenic tobacco.