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目的:以朝鲜白头翁种子为供试材料,筛选并确定调控朝鲜白头翁种胚快速发育和种子萌发的主要因素及水平。方法:通过多因子交叉重复试验对影响朝鲜白头翁种胚发育和种子萌发的主要因素及其水平进行探讨。结果:朝鲜白头翁种胚发育成熟和种子萌发的方法为:种子用2.40 mg/L KT、2.80 mg/L GA3和0.30~0.70mg/L 2,4-D混合溶液浸泡24 h,将种子与河砂(1∶2)混合,进行63~70 d的变温处理,变温幅度和时间:白天温度为(23±2)℃,处理14 h,夜间温度为(10±2)℃,处理10 h,子叶胚发生率95.1%,种子发芽率92.3%。结论:解决了朝鲜白头翁种胚发育调控和育苗技术,该技术适合于朝鲜白头翁种苗的工厂化生产。
OBJECTIVE: To screen and identify the main factors and levels that regulate the rapid development of embryos and seed germination of Pulsatilla wallichiana in North Korea. Methods: The main factors influencing the embryo development and seed germination of Pulsatilla chinensis were studied by multi-factor cross-repeat test. Results: The seed germination and maturation of Pulsatilla chinensis were as follows: The seeds were soaked in 2.40 mg / L KT, 2.80 mg / L GA3 and 0.30 ~ 0.70 mg / L 2,4-D mixed solution for 24 h, (1: 2) for 63 ~ 70 d. The temperature range and time were: the daytime temperature was 23 ± 2 ℃, the treatment time was 14 h, the nighttime temperature was 10 ± 2 ℃, The incidence of cotyledons embryo 95.1%, seed germination rate 92.3%. Conclusion: The solution to the embryonic developmental regulation and seedling raising of Pulsatilla korea is solved. This technique is suitable for the industrialized production of Pulsatilla chinensis seedlings.