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对大多数肿瘤活检标本,可采用组织病理学方法加以确诊,但有些病例依上法常难以下诊断或存有争议。为此,作者运用免疫组织学技术对120例经常规组织病理学方法无法确诊的肿瘤患者(其中分类不明的24例,疑似癌和淋巴瘤者分别为43和53例)进行了研究.标本在作石蜡包埋前均以甲醛盐液固定,并再由12例患者获取未固定组织,以作冰冻切片.所用单克隆抗体包括 PD7/26、2B11、E2g、CEA 和 KLI 等,并率先以免疫细胞化学的方法对其特异性分别作了确
For most tumor biopsy specimens, histopathological methods can be used for diagnosis, but some cases are difficult to diagnose or controversial according to the method. To this end, the authors used immunohistochemical techniques to study 120 cases of tumor patients that could not be diagnosed by conventional histopathological methods (24 of them were unclassified, and 43 and 53 were suspected cancer and lymphoma, respectively). Before paraffin embedding, they were fixed with formaldehyde solution, and unfixed tissues were obtained from 12 patients for frozen sections. The monoclonal antibodies used included PD7/26, 2B11, E2g, CEA, and KLI, and were first immunized. The method of cytochemistry was used to confirm their specificity.