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目的:观察硫酸脑苷酯对哮喘小鼠气道炎症及IL-10的影响,探讨其在哮喘治疗中可能的机制。方法:将26只6-8周龄的BALB/c雌性小鼠随机分成A、B、C组,A组为正常对照组(6只),B组为哮喘对照组(10只),C组为硫酸脑苷酯干预的哮喘组(10只)。应用苏木精-伊红(HE)染色行肺组织病理学检查。实时荧光定量聚合酶链反应法(Real-timePCR)检测肺组织中白细胞素-10(IL-10)mRNA水平表达。酶联免疫吸附法(ELISA)测定血清中IL-10的水平。结果:病理组织学显示C组较B组肺组织炎性细胞浸润明显减少。B组和C组的肺组织和血清中IL-10的表达水平均低于A组(均为P<0.01)。予以硫酸脑苷酯干预后,其肺组织和血清中IL-10的表达水平均高于B组(P<0.01;P<0.05)。结论:①哮喘的发生与IL-10表达降低有关;②硫酸脑苷酯可减轻哮喘小鼠气道炎症,其机制可能与促进IL-10的产生有关。
Objective: To observe the effects of cerebroside on airway inflammation and IL-10 in asthmatic mice and to explore its possible mechanism in the treatment of asthma. Methods: Twenty-six BALB / c female mice aged 6-8 weeks were randomly divided into A, B and C groups. A group was normal control group (6 mice), B group was asthma control group (10 mice), C group Asthmatic mice treated with cerebroside (10 mice). Histopathological examination of hematoxylin and eosin (HE) was performed. Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of interleukin-10 (IL-10) mRNA in lung tissue. Serum IL-10 levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: Histopathology showed that the infiltration of inflammatory cells in lung tissue in group C was significantly lower than that in group B. The expression of IL-10 in lung tissue and serum of group B and C were lower than that of group A (both P <0.01). The levels of IL-10 in lung tissue and serum were significantly higher than those in group B after intervention of cerebroside (P <0.01; P <0.05). Conclusion: (1) The occurrence of asthma is related to the decrease of IL-10 expression; (2) Cerebrolysine sulfate can reduce airway inflammation in asthmatic mice, which may be related to the promotion of IL-10 production.