论文部分内容阅读
以5个骨干玉米自交系幼胚为材料,研究幼胚大小、2,4-D浓度、KT浓度对幼胚愈伤组织诱导及分化的影响。结果表明,5个骨干玉米自交系均可诱导出愈伤组织,但不同条件下诱导率存在显著差异。最适幼胚大小为2.0～3.0 mm,因基因型不同而有所变化;2,4-D浓度在1.0～3.0 mg/L有利于愈伤组织诱导,同一材料不同2,4-D浓度对胚性愈伤组织诱导率的影响具有显著差异,不能以初级愈伤诱导率高低作为选择胚性愈伤诱导率的标准;KT可显著提高愈伤组织的分化率,随着KT浓度的增加分化率呈先上升后下降的趋势,最适KT浓度为1.0～2.5 mg/L。
The effects of immature embryo size, 2,4-D concentration and KT concentration on the callus induction and differentiation of immature embryos were studied by using the embryos of 5 maize inbred lines as material. The results showed that callus was induced in all five maize inbred lines, but there were significant differences in the induction rate under different conditions. The optimal embryo size was 2.0-3.0 mm, which varied with different genotypes. The concentration of 2,4-D at 1.0-3.0 mg / L was beneficial to callus induction. Different concentrations of 2,4-D Embryogenic callus induction rate of significant differences, can not be the primary callus induction rate as the selection of embryogenic callus induction rate; KT can significantly improve the differentiation rate of callus, with the KT concentration increased differentiation The rate was first increased and then decreased, the optimum KT concentration of 1.0 to 2.5 mg / L.