论文部分内容阅读
目的:研究二色补血草多糖羧甲基化的最优合成工艺。方法:运用响应面法,以多糖羧甲基化的取代度为评价指标,考察反应时间、氯乙酸浓度和反应温度对多糖羧甲基化的取代度的影响。结果:通过响应面法得到的最优工艺条件为:反应时间4.0 h、氯乙酸浓度是2.8 mol/L、反应温度45℃,在此条件下,二色补血草多糖羧甲基化的取代度为0.853。初步的抗氧化性试验表明,羧甲基化后的二色补血草多糖的抗氧化性能有明显提高。结论:优化得到的二色补血草多糖的羧甲基化工艺路线可行,对进一步拓宽其应用具有一定的参考价值。
Objective: To study the optimal synthesis of carboxymethylation of two-color Limonella polysaccharide. Methods: The response surface methodology was used to evaluate the degree of carboxymethylation of polysaccharides. The effects of reaction time, concentration of chloroacetic acid and reaction temperature on the degree of carboxymethylation of polysaccharides were investigated. Results: The optimal reaction conditions were as follows: the reaction time was 4.0 h, the concentration of chloroacetic acid was 2.8 mol / L and the reaction temperature was 45 ℃. Under these conditions, the degree of carboxymethyl substitution Is 0.853. Preliminary anti-oxidative tests showed that the oxidation resistance of carboxymethyl methylated polysaccharides increased significantly. Conclusion: The carboxymethyl methylation process of the optimized two - color Limonium polysaccharide is feasible and has certain reference value for further widening its application.