建立了光度测定酶法合成中色氨酸含量的新体系。该检测体系不仅能准确地测定产物色氨酸的含量，且对底物吲哚能同时测定。有效地避免了其他色氨酸测定方法底物吲哚的干扰，通过标准加入法色氨酸及吲哚的回收率分别为９７．８％～１０３．３％，９７．６％～１０３％。实验证明该体系简单、实用，测定结果可靠。 A new system of photometric determination of tryptophan in enzymatic synthesis was established. The detection system can not only determine the content of tryptophan accurately, but also determine the indole of substrate at the same time. Interference of indole, which is the substrate for other tryptophan assays, was effectively avoided. The recoveries of the tryptophan and indole by standard addition method were 97.8% -103.3% and 97.6% -103%, respectively. Experiments show that the system is simple and practical, and the results are reliable.