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目的:研究cyclin D1,bcl-2,p53和survivin在丙型肝炎病毒(hepatitis C virus,HCV)相关性肝细胞性肝癌(hepatocellular carcinoma,HCC)癌组织及癌旁组织中的表达,探讨其表达与丙型肝炎病毒相关性肝细胞性肝癌患者临床病理特征及生存预后之间的关系。方法:采用免疫组化检测方法检测cyclin D1,bcl-2,p53和survivin在丙型肝炎病毒相关性肝癌组织、癌旁组织和基本正常的肝组织中的表达,统计分析各因子的表达情况以及与患者临床病理特征的关系,并利用Kaplan-Meier分析法进一步分析各因子与患者生存预后之间的关系。结果:cyclin D1,bcl-2,p53和survivin在基本正常肝脏组织、癌旁组织和癌组织中的表达呈现递增的趋势,且cyclin D1,bcl-2,p53和survivin在癌组织的表达显著高于癌旁组织和基本正常的肝脏组织(P<0.05);经统计学分析,cyclin D1,bcl-2和p53与肿瘤的分化程度相关(P<0.05),而survivin与血管的浸润情况相关(P<0.05);Kaplan-Meier分析显示,cyclin D1,p53和survivin与患者的不良预后有关(P<0.05),而bcl-2与患者的不良预后无关(P>0.05)。结论:cyclin D1,bcl-2,p53和survivin可能与丙型肝炎病毒相关性肝细胞性肝癌的发生存在一定的联系,除此之外,cyclin D1,p53和survivin与丙型肝炎病毒相关性肝细胞性肝癌患者的不良预后相关,而bcl-2与预后不存在显著相关性。
OBJECTIVE: To investigate the expression of cyclin D1, bcl-2, p53 and survivin in the tissues of hepatocellular carcinoma (HCC) and their adjacent tissues of hepatitis C virus (HCV) And hepatitis C virus associated hepatocellular carcinoma in patients with clinicopathological features and prognosis of the relationship. Methods: The expressions of cyclin D1, bcl-2, p53 and survivin in hepatitis C virus-associated hepatocellular carcinoma tissues, paracancerous tissues and normal liver tissues were detected by immunohistochemistry. The expression of each factor was statistically analyzed And the relationship between the clinicopathological features and the use of Kaplan-Meier analysis of further analysis of the relationship between factors and prognosis of patients. Results: The expressions of cyclin D1, bcl-2, p53 and survivin in normal liver tissue, paracancerous tissue and cancer tissues showed an increasing trend, and the expression of cyclin D1, bcl-2, p53 and survivin in cancer tissues was significantly higher (P <0.05). The levels of cyclin D1, bcl-2 and p53 were correlated with tumor differentiation (P <0.05), while survivin was correlated with vascular invasion (P <0.05) P <0.05). Kaplan-Meier analysis showed that cyclin D1, p53 and survivin were correlated with poor prognosis (P <0.05), while bcl-2 was not associated with poor prognosis (P> 0.05). Conclusions: Cyclin D1, bcl-2, p53 and survivin may be related to the occurrence of hepatitis C virus associated hepatocellular carcinoma. In addition, cyclin D1, p53 and survivin are associated with hepatitis C virus associated liver The poor prognosis of patients with hepatocellular carcinoma was correlated, while there was no significant correlation between bcl-2 and prognosis.