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[目的]分离并鉴定棘胸蛙歪头病病原。[方法]从患歪头病棘胸蛙(Quasipaa spinosa)体内分离到致病菌,分别采用4种不同方式进行人工感染试验,通过形态观察、生理生化试验、16S r DNA测序等方法进行鉴定,并采用抑菌圈研究该菌株对12种抗生素的敏感性。[结果]除口服方式外,肌肉注射、皮肤损伤浸泡、皮肤不损伤浸泡感染途径均能使棘胸蛙发病并导致死亡,且对蛙有较强的致病性。通过生理生化试验和16S r DNA测序等方法鉴定致病菌为脑膜炎败血金黄杆菌(Chryseobacterium meningosepticum)。药敏试验结果表明:该致病菌对万古霉素、头孢哌酮、庆大霉素和红霉素高度敏感,而对吡哌酸、链霉素、四环素、诺氟沙星、青霉素、新霉素、林可霉素、先锋霉素不敏感。[结论]棘胸蛙歪头病病原为脑膜炎败血金黄杆菌。
[Objective] To isolate and identify the pathogen of spinous tree frog head tilt disease. [Method] Pathogenic bacteria were isolated from Quasipa spinosa, suffering from head tilt disease. Four kinds of artificial infection tests were conducted respectively. The morphological, physiological and biochemical tests and 16S r DNA sequencing were used to identify the pathogens. The antibacterial circle was used to study the sensitivity of the strain to 12 antibiotics. [Result] Except for oral administration, intramuscular injection, skin damage and soaking, and the skin does not damage the soaking infection route can lead to the occurrence and death of Spinach frog, and have strong pathogenicity to frogs. The pathogen was identified as Chryseobacterium meningosepticum by physiological and biochemical tests and 16S r DNA sequencing. Susceptibility test results show that: the pathogen is highly sensitive to vancomycin, cefoperazone, gentamicin and erythromycin, while for pipemidic acid, streptomycin, tetracycline, norfloxacin, penicillin, new Mycotoxin, lincomycin, cephalosporin insensitive. [Conclusion] The pathogen of spinous tree frog head disease is meningitis.