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目的 探讨纤溶酶原活化剂抑制物 - 1(plasminogen activator inhibitor- 1,PAI- 1)基因和纤维蛋白原β链基因多态性与狼疮性肾炎 (lupus nephritis,L N)肾小球微血栓形成之间的相关性。方法 选取10 1例 L N患者 ,依据肾活检组织肾小球微血栓的有无 ,将患者分为两组 :L N伴血栓 (L N+T)组 46例 ;不伴血栓 (L N- T)组 5 5例。应用聚合酶链反应 -限制性片段长度多态性和聚合酶链反应 -序列长度多态性技术分别分析两候选基因的基因型。正常对照组为 12 8名健康成人。结果 (1) PAI- 1基因 4G/ 4G基因型和4G等位基因与 L N+T组显著相关 ;L N中 4G/ 4G型患者发生肾小球袢内血栓的相对风险率的比值比(odds ratio,OR)为 2 .96 ,95 %可信区间 (confidence interval,CI) :1.2 6～ 6 .92 ;(2 )纤维蛋白原 β链基因G/ A+A/ A基因型和 A型等位基因与 L N+T组显著相关 ;L N中 A型等位基因携带者发生肾小球袢内血栓的相对风险率为 OR=2 .44 ,95 % CI:0 .98～ 5 .5 9;(3) L N患者若同时兼有上述两基因的血栓易感基因型 ,其发生肾小球微血栓的相对风险率明显增加 ,OR=4.5 ,95 % CI:1.34～ 15 .12 ;血栓易感基因型的混合病因分值 (4 5 .98% )也高于各自单独的病因分值 (PAI- 1基因 4G/ 4G型为 31.6 7%、纤维蛋白原 β链
Objective To investigate the relationship between polymorphism of plasminogen activator inhibitor-1 (PAI-1) gene and fibrinogen β chain gene and glomerular microthrombosis in lupus nephritis (LN) The correlation between. Methods Totally 101 patients with LN were divided into two groups according to the presence or absence of glomerular microthrombi in the renal biopsy: 46 patients with LN + T (LN + T) Group 55 cases. The genotypes of two candidate genes were analyzed by polymerase chain reaction-restriction fragment length polymorphism and polymerase chain reaction-sequence length polymorphism respectively. The normal control group was 12 8 healthy adults. Results (1) The 4G / 4G genotype and 4G allele of PAI-1 gene were significantly correlated with L N + T group. The odds ratio of relative risk of glomerular thrombosis in 4G / 4G type of LN patients (odds (OR) was 2.96, the 95% confidence interval (CI) was 1.2 6 ~ 6 .92; (2) The genotypes of G / A + A / A and A The relative risk of thrombosis in patients with type A allele of LN was OR = 2.44, 95% CI: 0.98-5.59 ; 3. The relative risk of glomerular micro-thrombosis in patients with LN was significantly higher than that in patients with both types of thrombosis (OR = 4.5, 95% CI: 1.34-15.15) The mixed etiology score of sensory genotype (45.98%) was also higher than that of their respective etiologies (PAI-1 gene 4G / 4G was 31.6 7%, fibrinogen beta chain