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本文介绍含有炔诺酮的炔雌醇片,采用萤光分光法测定炔雌醇的含量,最低检出量为10μg/ml,最佳测定范围为17.5～280μg/ml,一般片剂的赋形药以及炔诺酮含量即使高达2000μg/ml对本测定也无干扰,可应用于片剂释放度试验中释放量的测定。测定方法标准曲线:取标准溶液(炔雌醇氯仿溶液3.5μg/ml)0、0.125、0.25、0.5、1.0和2.0ml,分别置于具螺帽的试管中,用氮驱去氯仿,残留物中加入4%氢氧化钠-乙醇溶液(氢氧化钠4g溶于10%乙醇100ml中)0.5ml,然后加入80%硫酸2ml,混和,放置30分
This article describes ethinyl estradiol tablets containing norethindrone, ethinyl estradiol content determined by fluorescence spectrometry, the minimum detectable amount of 10μg / ml, the best determination of the range of 17.5 ~ 280μg / ml, the general shape of tablets Even if the drug and norethindrone level are as high as 2000 μg / ml, this test is also free from interference and can be applied to the determination of the amount released in the tablet release test. Determination of the standard curve: Take standard solution (ethinylestradiol chloroform solution 3.5μg / ml) 0,0.125,0.25,0.5,1.0 and 2.0ml, respectively, placed in a test tube with a nut, with nitrogen to remove chloroform, the residue Added 4% sodium hydroxide - ethanol solution (sodium hydroxide 4g dissolved in 10% ethanol 100ml) 0.5ml, and then added 80ml sulfuric acid 2ml, mixed and placed 30 minutes