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目的:研究生殖器疱疹(GH)患者皮损部位单纯疱疹病毒-Ⅱ(HSV-2)载量和外周血细胞免疫功能的相关性。方法:采用荧光定量聚合酶链反应(fluorescent quantitative polymerase chain reac-tion,FQ-PCR)定量检测28例现症GH患者皮损部位HSV-2 DNA载量,采用流式细胞计数的方法监测28例现症GH患者外周血T淋巴细胞亚群、NK细胞及B细胞的比例,用线性相关和回归方法分析现症GH患者病毒载量与细胞免疫功能的相关性。结果:①GH患者皮损内疱液HSV-2检出率为100%,病毒载量为6190714±1962085拷贝/μg DNA。HSV-2病毒载量与患者复发次数呈显著正相关。②GH患者CD3+、CD4+、CD5+6细胞数及CD4+/CD8+比值均较对照组低(P<0.01),但B细胞数与对照组相比差异无统计学意义(P>0.05)。GH患者皮损HSV-2病毒载量与患者外周血CD3+、CD4+、CD8+、NK、CD4+/CD8+细胞呈显著的负相关。结论:GH患者的细胞免疫功能抑制可能是由于外周血T淋巴细胞亚群的变化和NK细胞减少,以及Th及Tc细胞亚群分化失衡造成,这种状态不利于机体抑制病毒复制和增殖,造成GH反复发作。
Objective: To study the correlation between the herpes simplex virus-Ⅱ (HSV-2) load and the immune function of peripheral blood in patients with genital herpes (GH). Methods: The quantitative polymerase chain reaction (fluorescent quantitative polymerase chain reac-tion, FQ-PCR) HSV 2-DNA load 28 cases are symptoms of skin lesions in patients with GH quantitative detection, flow cytometry using the method of monitoring 28 cases The incidence of GH patients with peripheral blood T lymphocyte subsets, NK cells and B cells in the proportion of linear correlation and regression analysis of the present disease GH patients with viral load and cellular immune function. Results: ① The detection rate of HSV-2 in blistering lesions of GH patients was 100%, and the viral load was 6190714 ± 1962085 copies / μg DNA. There was a significant positive correlation between HSV-2 viral load and the number of patients relapsed. ② The ratio of CD3 +, CD4 +, CD5 + 6 cells and CD4 + / CD8 + in patients with GH were lower than those in control group (P <0.01). However, the number of B cells was not significantly different from that in control group (P> 0.05). The HSV-2 viral load in patients with GH was significantly and negatively correlated with CD3 +, CD4 +, CD8 +, NK and CD4 + / CD8 + cells in peripheral blood of patients with GH. CONCLUSIONS: The suppression of cellular immune function in GH patients may be due to the changes of T lymphocyte subsets and NK cells in peripheral blood and imbalance of Th and Tc cell subsets, which is not conducive to the inhibition of virus replication and proliferation in the body, resulting in GH recurrent.