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目的研究顺铂联合8-硝基白杨素对人卵巢癌COC1细胞裸鼠移植瘤生长的影响。方法建立人卵巢癌COC1细胞裸鼠皮下移植瘤模型,随机分成4组,每组5只:生理盐水组、8-硝基白杨素组、顺铂组、8-硝基白杨素+顺铂组。观察各组裸鼠移植瘤体积、重量及裸鼠体质量的变化;裸鼠血清乳酸脱氢酶、谷丙转氨酶、肌酐值和外周血白细胞计数的变化;FCM测定瘤组织细胞凋亡率;Western blot分析瘤组织细胞凋亡的可能分子生物学机制。结果与顺铂组和8-硝基白杨素组相比,顺铂联合8-硝基白杨素联合作用COC1细胞裸鼠移植瘤后,移植瘤的体积、移植瘤的重量均明显降低(P<0.05);与顺铂组相比,顺铂联合8-硝基白杨素联合组裸鼠体质量无明显差异(P>0.05),裸鼠血清乳酸脱氢酶、谷丙转氨酶、肌酐值和外周血白细胞计数无明显差异(P>0.05);顺铂和8-硝基白杨素联合作用COC1细胞16 d后,COC1细胞发生凋亡,同时bcl-2蛋白表达降低,caspase-3蛋白表达增高。结论顺铂联合8-硝基白杨素通过降低bcl-2蛋白表达,活化caspase-3蛋白表达来抑制人卵巢癌COC1细胞裸鼠移植瘤生长。
Objective To study the effects of cisplatin combined with 8-nitrocyanin on the growth of transplanted human ovarian cancer COC1 cells in nude mice. Methods Human ovarian cancer COC1 cells subcutaneously transplanted in nude mice model were established and randomly divided into 4 groups with 5 rats in each group: saline group, 8-nitrocyanin group, cisplatin group, 8-nitrocyanin + cisplatin group . The changes of the nude mice tumor volume and weight and nude mice body weight were observed. The changes of serum lactate dehydrogenase, alanine aminotransferase, creatinine and leukocyte count in nude mice were observed. The apoptosis rate of tumor cells was determined by FCM blot analysis of possible molecular biological mechanism of tumor cell apoptosis. Results Compared with cisplatin group and 8-nitrocyanin group, the transplanted tumor volume and tumor weight of COC1 cells treated with cisplatin combined with 8-nitro-chrysin were all significantly decreased (P < 0.05). Compared with the cisplatin group, there was no significant difference in the body weight between the cisplatin and 8-nitrocyanin combination groups (P> 0.05). The levels of serum lactate dehydrogenase, alanine aminotransferase, (P> 0.05). COC1 cells apoptosis occurred in COC1 cells treated with cisplatin and 8-nitrocyanin for 16 days, while the expression of bcl-2 protein and caspase-3 protein increased. Conclusion Cisplatin combined with 8-nitro-chrysin can inhibit the growth of human ovarian cancer COC1 cells in nude mice by decreasing the expression of bcl-2 protein and activating caspase-3 protein expression.