目的建立清开活血片微生物限度检查方法。方法采用常规法和培养基稀释法测定清开活血片对大肠埃希菌等5种试验菌株的回收率,并验证了检查控制菌大肠埃希菌、大肠菌群和沙门菌的方法。结果清开活血片对金黄色葡萄球菌有抑制作用,采用培养基稀释法可消除其抑制作用。清开活血片对大肠埃希菌、枯草芽孢杆菌、白色念珠菌和黑曲霉菌无抑制作用。结论采用常规法和培养基稀释法可准确检出清开活血片的微生物污染程度。 Objective To establish a microbial limit test method for Qingkaohuoxue tablets. Methods The conventional method and medium dilution method were used to determine the recovery rate of Qingkaihuoxue tablets against E. coli and other five test strains, and the methods for checking the control bacteria Escherichia coli, coliform bacteria and Salmonella were verified. Results Qingkaihuoxue had inhibitory effect on Staphylococcus aureus. The inhibition effect was eliminated by medium dilution method. Qingkai Huoxue Tablets had no inhibitory effect on Escherichia coli, Bacillus subtilis, Candida albicans, and Aspergillus niger. [Conclusion] The degree of microbial contamination of Qingkai Huoxue Tablets can be accurately detected by conventional method and medium dilution method.