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神经营养因子(NTFs)在神经系统发育、正常功能维持和损伤修复中起着极其重要的作用.本研究用纯化培养的运动神经元鉴定了人胚骨骼肌提取液的运动神经元营养活性,用分子生物学等方法从人胚骨骼肌提取液中初步分离纯化出运动神经营养成分.研究结果表明:人胚骨骼肌抽取液能维持运动神经元存活约1～2周,促进突起生长,而对照组的运动神经元在3天左右迅速死亡.人胚骨骼肌提取液和脊髓非运动神经元对运动神经元的营养作用有协同性,共同维持运动神经元存活2—3周.通过用鼠胚骨骼肌抽取液对照培养,表明人隔骨骼肌提取液中也含有运动神经元营养物质,其作用受种属限制不明显,最佳效应浓度为100μg/ml.人胚骨骼肌提取液经硫酸铵分级盐析、Sephcry S-200分子筛等蛋白纯化过程.每步纯化所得样品经多
Neurotrophic factors (NTFs) play an important role in nervous system development, normal function maintenance and injury repair.In this study, the purified cultured motor neurons were identified in human embryonic skeletal muscle motility of neuronal nutrition, with Molecular biology and other methods from the human embryonic skeletal muscle extract of the initial separation and purification of motor nerve nutrients.The results show that: human embryonic skeletal muscle extract can maintain the survival of motor neurons for about 1 to 2 weeks, and promote the growth of the control, while the control Group of motor neurons died rapidly in about 3 days.Human embryonic skeletal muscle extract and spinal cord non-motor neurons of motor neurons nutrition synergies to maintain the motor neuron survival for 2-3 weeks by using mouse embryos Skeletal muscle extract control culture, indicating that the human skeletal muscle extract also contains motor neuron nutrients, the role of species constraints is not obvious, the optimal concentration of 100μg / ml human embryonic skeletal muscle extract by ammonium sulfate Fractional salting out, Sephcry S-200 molecular sieve and other protein purification process.